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《阻斷shh信號(hào)通路對(duì)膠質(zhì)細(xì)胞炎性激活的實(shí)驗(yàn)研究》由會(huì)員上傳分享,免費(fèi)在線閱讀,更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、廣州醫(yī)學(xué)院碩士研究生學(xué)位論文阻斷SHH信號(hào)通路對(duì)膠質(zhì)細(xì)胞炎性激活的實(shí)驗(yàn)研究EffectofblockingtheSHHsignalingpathwayoninflammation-activedofgliomacell專業(yè)名稱:人體解剖與組織胚胎學(xué)學(xué)位申請(qǐng)人:王迪學(xué)號(hào):2009211957指導(dǎo)老師:洪樂鵬副教授廣州醫(yī)學(xué)院廣州二0一二年三月目錄摘要.............................................................................................................
2、...................1Abstract..........................................................................................................................4中英文對(duì)照表......................................................................................................7前言.....................
3、.......................................................................................................8第一部分LN229細(xì)胞炎癥激活模型的建立及阻斷SHH通路對(duì)炎癥因子表達(dá)的影響..............................................................................................................................111實(shí)驗(yàn)材料........
4、................................................................................................111.1實(shí)驗(yàn)細(xì)胞........................................................................................................111.2實(shí)驗(yàn)主要試劑...............................................................
5、.................................111.3實(shí)驗(yàn)儀器........................................................................................................111.4主要試劑的配制............................................................................................121.5PCR引物.......................
6、................................................................................122實(shí)驗(yàn)方法........................................................................................................132.1細(xì)胞培養(yǎng)...................................................................................
7、.....................132.2細(xì)胞處理及分組............................................................................................142.3CCK-8法檢測(cè)細(xì)胞存活...............................................................................142.4實(shí)時(shí)熒光定量PCR................................................
8、...................