資源描述:
《黃連、大黃、牡丹皮三種植物對松材線蟲的毒殺活性與作用機(jī)理分析》由會員上傳分享,免費(fèi)在線閱讀,更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫。
1、theonsetof‘'water+nematode’’treatmentwithin10days.Butpinesof“water+nematode”treatmentbegantogetdisease,anddiseaseindexraserapidlyafter10days.Inend,thepinesgotwiltandevendiedwithin40days.Anatherobjectiveofthisstudyistoidentifypoisoningmechanismofethanolextractsofthreetradit
2、ionalChinesemedicinal(TCM)herbs:Cortexmoutanradicis,CoptischinensisfranchandRadixetrhizomarhei.InhibitoryactivityofextractsofthreeTCMherbs011myceliagrowthofCordycepssinensiswasscreenedbyusingmyceliaradialgrowthinhibitionmethod.Theeffectsoftheseextractswereevaluatedonmetabo
3、lismoftotalsugarandproteinandactivitiesofprotectiveenzymes:Superoxidedismutase(SOD),Catalase(CAT),Peroxidase(POD),Glutathioneperoxidase(OSH—Px),Acetylcholinesteras(AChE)andcontentofMalondialdehyde(MDA)fromBursaphelenchusxylophilus.AnalyzingofgeneexpressivequantityofSOD,CAT
4、,GSH-PxandAChEwasmadebywestemblot.TheextractsofthreeTCMherbsstronglyinhibitedmyceliagrowth,interferedmetabolismofsugarandproteinof丑xylophilus.TheenzymeactivitiesofGSH—Px.CAT,andSODwereimproved;theenzymeactivityofPODwasinhibited.TheMDAcontentWasincreased.Oxidationandantioxi
5、dantwereimbalanceandgreatlyaffectedcellfunctions.TheactivityofAChEWasinhibited,SOnervoussystemWasdamaged.Itisconcludedthatchangesofsomephysio—biochemicalindexesofB.xylophilusinvivocausedbyextractsofthreeTCMherbsweremaincausesofitsdeath.KeyWords:Cortexmoutanradicis,Coptisch
6、inensisfran以Radixetrhizomarhei,Bursaphelenchusxylophilus,nematicidalactivity,nematicidaimechanismVI目錄摘要???????????????????????????????IIIAstract................。.。.................。。..........。.。.。.。..。.。。..。。。。.。..。.。.。.。。..。....。。,,117’目錄???????????????????????????????Ⅶ緒
7、{倉??????????????????????????????????????????.1第1章文獻(xiàn)綜述??????????????????????????31.1植物寄生線蟲????????????????????????..31.1。1植物寄生線蟲概述????????????????????31.1.2植物寄生線蟲分類????????????????????31.2植物寄生線蟲的防治?????????????????????.41.2.1化學(xué)防治植物寄生線蟲??????????????????41.2.2植物源防治植物寄生線蟲????
8、?????????????51.3松材線蟲病及其防治?????????????????????..61.3.1松材線蟲病的發(fā)現(xiàn)及其病害????????????????61.3.2