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1、山西醫(yī)科大學(xué)碩士學(xué)位論文1．rhG-CSF治療腦缺血再灌注損傷大鼠，可改善早期神經(jīng)功能缺損，促進(jìn)肢體功能恢復(fù)。2．rhG-CSF治療降低了腦缺血再灌注后MMP-3及MMP-9的表達(dá)，并降低了血腦屏障通透性，從而減輕腦水腫，這可能是rhG-CSF的腦保護(hù)作用機(jī)制之一。關(guān)鍵詞重組人粒細(xì)胞集落刺激因子；腦缺血再灌注；基質(zhì)金屬蛋白酶-3；基質(zhì)金屬蛋白酶-9；血腦屏障2萬(wàn)方數(shù)據(jù)山西醫(yī)科大學(xué)碩士學(xué)位論文TheeffectofrhG-CSFontheexpressionofMMP-3andMMP-9proteinfollowi
2、ngfocalcerebralischemia-reperfusioninjuryinratsAbstractObjectiveToexploretheneuralprotectionmechanismofG-CSFbyobservingthechangeofexpressionofMMP-3、MMP-9andthepermeabilityofblood-brainbarrierinSDratswithcerebralischemia-reperfusioninjurythattreatedwithrecombi
3、nanthumangranulocytecolony-stimulatingfactor（rhG-CSF）.MethodsThirty-nineSprague-Dawleymaleratswererandomlydividedinto3groups,sham-operatedgroup,middlecerebralarteryocclusion/reperfusion(MCAO/R)groupandrhG-CSFtreatmentgroup,with13ratsineachgroup.Establishingmi
4、ddlecerebralarteryocclusionreperfusionratsmodelbylongalineplugmethod.Dissectinginternalcarotidarteryandexternalcarotidarteryinsham-operatedgroup.TherhG-CSFtreatmentgroupreceivedadoseof50ug/kgofrhG-CSFwhichwasintraperitonealinjectedattheonsetofreperfusionafter
5、2hocclusionand24hafterreperfusion.Whilesham-operatedgroupandMCAO/Rgroupwereinjectedthesamevolumeofsaline.Longa5-pointscalewasadoptedtoscoreneurologicalfunctionat48hafterthereperfusion.TheexpressionofMMP-3andMMP-9weredetectedbyimmunohistochemicalmethod.Thecont
6、entofEvenblueinthebrainweredetectedbymicroplatereader.Transmissionelectronmicroscopewasusedtoobservetheultrastructureofblood-brainbarrier.Result1.TheMCAO/RgroupandrhG-CSF-treatmentgrouphaddifferentdegreesoflimbparalysisaftercerebralischemiareperfusion．Compare
7、dwiththeMCAO/Rgroup，theneurologicalfunctionscoresoftherhG-CSF-treatmentgroupreducedobviouslyandthementalstateimproved，thedifferencewasstatisticallysignificant(P<0．05).2.Comparedwithsham-operatedgroup,theexpressionofMMP-3andMMP-9positivecellsweresignificantlyi
8、ncreasedintheischemicacore(striatumandcortex),greyvaluedecreasedintheMCAO/RgroupandrhG-CSF-treatmentgroup(P<0．05).ComparedwiththeMCAO/Rgroup,theexpressionofMMP-3andMMP-9positivecellsweres
