水稻耐淹澇性狀的遺傳分析和ssr標(biāo)記的研究

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1、遺傳HEREDITAS(Beijing)28(12):1562~1566,2006研究報(bào)告DOI:10.1360/yc-006-1562水稻耐淹澇性狀的遺傳分析和SSR標(biāo)記的研究1~32312陳永華,趙森,柳俊,嚴(yán)欽泉,肖國櫻(1.湖南農(nóng)業(yè)大學(xué)農(nóng)學(xué)院,長(zhǎng)沙410128;2.中國科學(xué)院亞熱帶農(nóng)業(yè)生態(tài)研究所,長(zhǎng)沙410125;3.中南林業(yè)科技大學(xué)資源與環(huán)境學(xué)院,長(zhǎng)沙410004)摘要:淹澇脅迫對(duì)水稻生產(chǎn)造成了嚴(yán)重影響,發(fā)掘可應(yīng)用于耐淹澇輔助選擇的分子標(biāo)記(MAS),將有助于水稻耐淹澇性狀的遺傳改良。應(yīng)用耐淹澇材料FR13A和淹澇敏感材料IR39595-503-2-1-2為親本做正反交獲得

2、F1和F2代群體。對(duì)正反交的F1群體的耐淹澇性狀進(jìn)行遺傳分析,發(fā)現(xiàn)正反交的F1代群體在耐淹澇性狀上沒有顯著差異,說明耐淹澇性狀是核基因控制。從兩次淹澇處理中F2代群體的分離情況來看,來源于FR13A的耐淹特性表現(xiàn)出數(shù)量-質(zhì)量性狀遺傳的特點(diǎn)。當(dāng)淹澇脅迫壓力比較輕時(shí)表現(xiàn)為數(shù)量性狀遺傳,具有微效多基因的作用。當(dāng)淹澇脅迫壓力增大時(shí),表現(xiàn)為主效基因控制的質(zhì)量性狀。在SSR分析中,187對(duì)SSR引物中有73對(duì)引物在兩親本間有明顯的差異,差異率為39%。用這73對(duì)差異引物,對(duì)F2群體進(jìn)行多態(tài)篩選,結(jié)果篩選到一個(gè)與耐淹澇性狀連鎖的標(biāo)記RM219,驗(yàn)證了耐淹澇性狀確實(shí)由主效基因Sub1控制,因此,RM

3、219在水稻耐淹澇育種中具有利用價(jià)值。關(guān)鍵詞:水稻;耐淹澇;SSR標(biāo)記中圖分類號(hào):Q953文獻(xiàn)標(biāo)識(shí)碼:A文章編號(hào):0253-9772(2006)12-1562-05GeneticAnalysisandSSRMarkerofTolerancetoSubmergenceinRice1~32312CHENYong-Hua,ZHAOSen,LIUJun,YANQin-Quan,XIAOGuo-Ying(1.HunanAgricultureUniversity,Changsha410128,China;2.InstituteofSubtropicalAgriculture,ChineseAca

4、demyofSciences,Changsha410125,China;3.CollegeofResourcesandEnvironment,CentralSouthUniversityofForestryandTechnology,Changsha410004,China)Abstract:Submergencestresshassevereharmtorice(OryzasativaL.)production.Itisimportanttoidentifymolecularmarkersassociatedwithtolerancetosubmergenceformarker-a

5、ssistedselection(MAS).F1andF2populationswareobtainedfromreciprocalcrossesbetweensubmergencetolerantparentFR13AandsubmergencesensitiveparentIR39595-503-2-1-2.NodifferenceinreciprocalcrossesinF1populationwasdetectedfortolerancetosubmergence.Thisindicatesthatthesubmergencecharacteriscontrolledbynu

6、cleicfactor(s).TolerancetosubmergencederivedfromFR13Aappearedtobeaquantitative-qualitativetraitasrevealedbythesegregationofF2populationintwosubmergentexperiments.Underlightsubmergencestress,multipleminorgenesmightworkandthetraitinheritedquantitativelyitappearedaquantitytrait,Whileunderseveresub

7、mergencestress,itexhibitedaqualitativeinheritancecontrolledbyamajorgene.Amongthe187pairsofSSRprimers,73(39%)werepolymorphicbetweenthetwoparentsandwereusedtotagsubmergenceintheF2population.PrimerRM219wasdetectedtobe收稿日期:2006-04-15;