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1、分類號：密級：HUAZHONGAGRICULTURALUNIVERSITY碩士學位論文MASTER’SDEGREEDISSERTATIONPEDV野毒株和疫苗毒株多重RT-PCR鑒別檢測方法的建立及應用ESTABLISHMENTANDAPPLICATIONOFMULTIPLEXRT-PCRMETHODFORIDENTIFICATIONOFPEDVWILDANDVACCINESTRAINS研究生:何東賢CANDIDATE:HEDONGXIAN學號:2016302120106STUDENTN:專業(yè):獸醫(yī)
2、碩士MAJOR:MASTERDEGREEINVETERINARYMEDICINE導師:何啟蓋教授SUPERVISOR:PROFESSORHEQIGAI湯細彪博士DOCTORTANGXIBIAO中國武漢WUHAN,CHINA二〇一八年六月JUNE,2018華中農(nóng)業(yè)大學碩士學位論文PEDV野毒株和疫苗毒株多重RT-PCR鑒別檢測方法的建立及應用EstablishmentandApplicationofMultiplexRT-PCRMethodforIdentificationofPEDVWildandV
3、accineStrains研究生:何東賢學號:2016302120106指導教師:何啟蓋教授湯細彪博士學位類型:獸醫(yī)碩士領(lǐng)域：華中農(nóng)業(yè)大學動物醫(yī)學院中國·武漢CollegeofVeterinaryMedince,HuazhongAgriculturalUniversityWuhan,ChinaPEDV野毒株和疫苗毒株多重RT-PCR鑒別檢測方法的建立及應用目錄摘要.....................................................................
4、...............................................................iAbstract...............................................................................................................................iii縮略語表(Abbreviation)................................
5、......................................................................v1前言..................................................................................................................................11.1豬流行腹瀉病毒(PEDV)...............................
6、............................................................21.1.1PEDV的病原學特征..........................................................................................21.1.2PEDV的基因組結(jié)構(gòu)......................................................................
7、....................31.1.3PEDV蛋白結(jié)構(gòu)和功能......................................................................................41.1.3.1復制酶多聚蛋白...........................................................................................41.1.3.2S蛋白............
8、................................................................................................41.1.3.3ORF3蛋白....................................................................................................41.1.3.4E蛋白...............
