異甘草素誘導(dǎo)人膠質(zhì)瘤shg44細(xì)胞自噬、凋亡并增強替莫唑胺抗膠質(zhì)瘤作用的研究

異甘草素誘導(dǎo)人膠質(zhì)瘤shg44細(xì)胞自噬、凋亡并增強替莫唑胺抗膠質(zhì)瘤作用的研究

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時間:2019-03-16

異甘草素誘導(dǎo)人膠質(zhì)瘤shg44細(xì)胞自噬、凋亡并增強替莫唑胺抗膠質(zhì)瘤作用的研究_第1頁
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1、分類號:密級:碩士研究生學(xué)位論文論文題目(中文)異甘草素誘導(dǎo)人膠質(zhì)瘤SHG44細(xì)胞自噬、凋亡并增強替莫唑胺抗膠質(zhì)瘤作用的研究論文題目(外文)TheStudyonIsoliquiritigeninInducedAutophagyandApoptosisofHumanGliomaCellsSHG44andEnhancedTemozolomideAnti-gliomaCells研究生姓名姜拓之學(xué)科、專業(yè)中西醫(yī)結(jié)合中西醫(yī)結(jié)合臨床研究方向中西醫(yī)結(jié)合防治神經(jīng)外科疾病導(dǎo)師姓名、職稱荔志云教授主任醫(yī)師論文工作起止年月2014年3月至201

2、5年2月論文提交日期2015年3月論文答辯日期2015年5月學(xué)位授予日期2015年6月獨創(chuàng)性聲明本人聲明所呈交的學(xué)位論文是本人在導(dǎo)師指導(dǎo)下進(jìn)行的研究工作及取得的研究成果。學(xué)位論文中凡引用他人已經(jīng)發(fā)表或未發(fā)表的成果、數(shù)據(jù)、觀點等,均已明確注明出處。除文中已經(jīng)注明引用的內(nèi)容外,不包含任何其他個人或集體已經(jīng)發(fā)表或撰寫過的科研成果。對本研究做出貢獻(xiàn)的個人和集體均已在論文中作了明確的說明并表示謝意。>論文作者簽名:簽字日期:/€年<月/日學(xué)位論文版權(quán)使用授權(quán)書本學(xué)位論文作者完全了解甘肅中醫(yī)藥大學(xué)有關(guān)保留、使用學(xué)位論文的規(guī)定,本人同意

3、學(xué)院保存或向國家有關(guān)部門或機構(gòu)送交論文的紙質(zhì)版和電子版,允許論文被查閱和借閱。本人授權(quán)甘肅中醫(yī)藥大學(xué)可以將學(xué)位論文的全部或部分內(nèi)容編入有關(guān)數(shù)據(jù)庫進(jìn)行檢索,可以采用任何復(fù)制手段保存、匯編學(xué)位論文。(保密的學(xué)位論文在解密后適用本授權(quán)書)學(xué)位論文作者簽名:^j簽字日期4以5年6月/日簽字日期年6月曰目錄中文摘要···················································································1ABSTRACT···················

4、····························································4中英文縮略詞表··········································································8前言·························································································9立題依據(jù)·························

5、··························································121.異甘草素抗膠質(zhì)瘤細(xì)胞的依據(jù)··················································122.異甘草素增強替莫唑胺抗膠質(zhì)瘤細(xì)胞作用的依據(jù)··························13論文正文··················································································

6、·14實驗一異甘草素抑制惡性膠質(zhì)瘤細(xì)胞的研究··································141材料··················································································141.1細(xì)胞系·········································································141.2實驗儀器和材料····························

7、·································142實驗方法···········································································172.1人膠質(zhì)瘤細(xì)胞SHG44的培養(yǎng)············································172.2細(xì)胞計數(shù)······································································182.3CC

8、K-8檢測異甘草素對SHG44膠質(zhì)瘤細(xì)胞的增殖抑制···········182.4流試細(xì)胞術(shù)分析異甘草素對SHG44細(xì)胞凋亡的影響··············182.5透射電鏡下觀測異甘草素對SHG44細(xì)胞超微結(jié)構(gòu)的改變········192.6免疫熒光觀察細(xì)胞中LC3B、Caspase-3蛋白的分布·

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