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《異甘草素誘導(dǎo)人膠質(zhì)瘤shg44細(xì)胞自噬、凋亡并增強替莫唑胺抗膠質(zhì)瘤作用的研究》由會員上傳分享,免費在線閱讀,更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、分類號:密級:碩士研究生學(xué)位論文論文題目(中文)異甘草素誘導(dǎo)人膠質(zhì)瘤SHG44細(xì)胞自噬、凋亡并增強替莫唑胺抗膠質(zhì)瘤作用的研究論文題目(外文)TheStudyonIsoliquiritigeninInducedAutophagyandApoptosisofHumanGliomaCellsSHG44andEnhancedTemozolomideAnti-gliomaCells研究生姓名姜拓之學(xué)科、專業(yè)中西醫(yī)結(jié)合中西醫(yī)結(jié)合臨床研究方向中西醫(yī)結(jié)合防治神經(jīng)外科疾病導(dǎo)師姓名、職稱荔志云教授主任醫(yī)師論文工作起止年月2014年3月至201
2、5年2月論文提交日期2015年3月論文答辯日期2015年5月學(xué)位授予日期2015年6月獨創(chuàng)性聲明本人聲明所呈交的學(xué)位論文是本人在導(dǎo)師指導(dǎo)下進(jìn)行的研究工作及取得的研究成果。學(xué)位論文中凡引用他人已經(jīng)發(fā)表或未發(fā)表的成果、數(shù)據(jù)、觀點等,均已明確注明出處。除文中已經(jīng)注明引用的內(nèi)容外,不包含任何其他個人或集體已經(jīng)發(fā)表或撰寫過的科研成果。對本研究做出貢獻(xiàn)的個人和集體均已在論文中作了明確的說明并表示謝意。>論文作者簽名:簽字日期:/€年<月/日學(xué)位論文版權(quán)使用授權(quán)書本學(xué)位論文作者完全了解甘肅中醫(yī)藥大學(xué)有關(guān)保留、使用學(xué)位論文的規(guī)定,本人同意
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4、····························································4中英文縮略詞表··········································································8前言·························································································9立題依據(jù)·························
5、··························································121.異甘草素抗膠質(zhì)瘤細(xì)胞的依據(jù)··················································122.異甘草素增強替莫唑胺抗膠質(zhì)瘤細(xì)胞作用的依據(jù)··························13論文正文··················································································
6、·14實驗一異甘草素抑制惡性膠質(zhì)瘤細(xì)胞的研究··································141材料··················································································141.1細(xì)胞系·········································································141.2實驗儀器和材料····························
7、·································142實驗方法···········································································172.1人膠質(zhì)瘤細(xì)胞SHG44的培養(yǎng)············································172.2細(xì)胞計數(shù)······································································182.3CC
8、K-8檢測異甘草素對SHG44膠質(zhì)瘤細(xì)胞的增殖抑制···········182.4流試細(xì)胞術(shù)分析異甘草素對SHG44細(xì)胞凋亡的影響··············182.5透射電鏡下觀測異甘草素對SHG44細(xì)胞超微結(jié)構(gòu)的改變········192.6免疫熒光觀察細(xì)胞中LC3B、Caspase-3蛋白的分布·