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1、華西口腔醫(yī)學雜志第26卷第2期2008年4月WestChinaJournalofStomatologyVol.26No.2Apr.2008·211·[文章編號]1000-1182(2008)02-0211-04絲素蛋白/納米羥磷灰石支架的實驗研究1211趙勇,李罡,陳靜,陳治清（1.口腔疾病研究國家重點實驗室,四川大學,四川成都610041;2.昆明醫(yī)學院口腔醫(yī)院修復科,云南昆明650031)[摘要]目的設計和構建三維絲素蛋白/羥磷灰石骨組織工程支架材料。方法聯(lián)合運用絲素蛋白非編織方法和仿生礦化技術，制備并表征三維多孔絲素蛋白/納米羥磷灰石的有機/無機組織工程支架。結果仿生礦化在非編織支架上
2、形成的針狀羥磷灰石晶體，直徑20～60nm，長100～300nm。復合支架孔隙度為70%～78%，孔徑為（163.4±42.6）μm。結論采用非編織絲素蛋白和仿生礦化的方法可制備孔隙度和孔徑可控的組織工程支架。[關鍵詞]絲素蛋白；仿生礦化；羥磷灰石[中圖分類號]R783.1[文獻標識碼]A1Preparationandcharacteristicsofnon-wovensilkfibroin/nano-hydroxyapatitescaffoldsZHAOYong，LI211Gang,CHENJing,CHENZhi-qing.（1.StateKeyLaboratoryofOralDisea
3、ses,SichuanUniversity,Chengdu610041,China;2.Dept.ofProsthodontics,StomatologicalHospitalofKunmingMedicalCollege,Kunming650031,China）[Abstract]ObjectiveTheaimofthepresentstudywastodesignandfabricateathreedimensiona（l3D）porousstructureofsilkfibroin/apatiteusedasapotentialscaffoldinbonetissueengineeri
4、ng.MethodsWiththecombininguseofnon-wovensilkfibroinnetandbiomimeticmethod,porousnon-wovensilkfibroin/nano-hydroxyapatitenet（NSF/nHAP）waspreparedandcharacterizedwithX-raydiffraction（XRD）,scanningelectronmicroscope（SEM）andfouriertransforminfraredspectroscopy（FTIR）.Theporosityandswellingratioofthe3Dsc
5、affoldwerealsomeasured.Besides,theosteoblastsfromthecraniumofnewbornSDratwereculturedonthepre-fabricatedscaffoldtoevaluatethebiologicalreactionofthescaffold.ResultsThenano-sizedhydroxyapatitecrystalswereneedle-likewiththelengthof100-300nmandthediameterof20-60nm.Thescaffoldfabricatedinthepresentstud
6、yexhibitedtheporousmicrostructurewithopenporosityaround70%-78%.Itsaverageporesizewasabou（t163.4±42.6）μm.Theswellingratioandwateruptakingwere4.56%and81.93%,respectively,whichrevealedthatthe3Dporousscaffoldhadanexcellenthydrophilicity.Therod-shapedapatitecrystalscouldrapidlyformonthesurfaceoffibroinf
7、ibersthroughoutthenetworkbyimmersingthenetintocalciumandphosphatesolutionsalternatively.ConclusionA3DporousNSF/nHAPscaffoldcanbefabricatedbybiomimeticmineralizationandnone-wovensilkfibroinmethod.ThenovelNSF
