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1、第29卷第3期西安交通大學學報(醫(yī)學版)Vol.29No.32008年6月JournalofXipanJiaotongUniversity(MedicalSciences)Jun.2008羥基喜樹堿對人膽管癌QBC939裸鼠移植瘤PS2、COX22的影響12223黃國棟,黃媛華,黃道富,肖美珍,唐麗君(1.南昌大學第一附屬醫(yī)院,江西南昌330006;2.湖南中醫(yī)藥大學附屬衡陽醫(yī)院,湖南衡陽421001;3.湖南省人民醫(yī)院,湖南長沙410002)摘要:目的探討羥基喜樹堿對人膽管癌QBC939裸鼠移植瘤的乳癌相關肽(PS2)、環(huán)氧合酶22(COX22)的影響,
2、分析PS2、COX22與腫瘤發(fā)生、發(fā)展的關系。方法建立人膽管癌QBC939細胞株裸鼠皮下移植瘤模型,將30只裸鼠隨機分成對照組、羥基喜樹堿低劑量組和羥基喜樹堿高劑量組,觀察移植瘤體生長情況,RT2PCR法檢測瘤體內PS2、COX22mRNA表達。結果與對照組比,羥基喜樹堿低、高劑量組都可使移植瘤體的PS2mRNA表達增高;COX22mRNA表達降低,且瘤體生長減慢,與對照組比較差異有統(tǒng)計學意義(P<0.01)。結論羥基喜樹堿可降低移植瘤COX22mRNA表達,增高移植瘤體的PS2mRNA表達,并使人膽管癌裸鼠移植瘤生長減慢。關鍵詞:膽管癌;裸鼠;移植;QB
3、C939細胞;PS2;COX22中圖分類號:R286.5文獻標識碼:A文章編號:167128259(2008)0320308203EffectofhydroxycamptothecineonPS2andCOX22ofthetransplantedtumorinthenudemicebyhumanbileductcarcinomaQBC939cells12223HuangGuodong,HuangYuanhua,HuangDaofu,XiaoMeizhen,TangLijun(1.theFirstAffiliatedHospitalofNanchangUni
4、versity,Nanchang330006;2.theAffiliatedHengyangHospital,HunanUniversityofTraditionalChineseMedicine,Hengyang421001;3.thePeoplepsHospitalofHunanProvince,Changsha410002,China)ABSTRACT:ObjectiveToinvestigatetheeffectofhydroxycamptothecineonPS2andCOX22innudemicebyhumanbileductcarcinoma
5、QBC939cellsinordertoanalyzethecorrelationbetweenPS2andCOX22expressionsandthedevelopmentofthetumor.MethodsAfterthemodelofthetransplantedtumorofsubcutaneoustissueslocatedthenudemicewasconstructedbyhumanbileductcarcinomaQBC939cells,30nudemicewererandomlydividedinto3groups,namely,cont
6、rolgroup,hydroxycamptothecinelow2dosegroupandhigh2dosegroup.ThegrowthofthetransplantedtumorwasobservedandimmunohistochemicalstainingcombinedwithimageanalysiswasusedtodeterminetheexpressionofPS2andCOX22inthetumortissues.ResultsComparedwiththatofthecontrolgroup,thecontentofPS2inthet
7、umortissueswasincreasedinhydroxycamptothecinelow2dosegroupandhigh2dosegroup.ThecontentofCOX22wasdecreasedandthegrowthofthetransplantedtumorwasinhibitedinbothhydroxycamptothecinelow2dosegroupandhigh2dosegroup(P<0.01).ConclusionHydroxycamptothecinecanreducethecontentsofCOX22andincre
8、asethecontentsofPS2inthetransplan
