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1、中國水稻科學(xué)(ChinJRiceSci),２０１６,３０(１):４４－５２http://www．ricesci．cn４４DOI:１０．１６８１９/j．１００１Ｇ７２１６．２０１６．５１２６秈稻９３Ｇ１１輻射誘變突變體庫的創(chuàng)建及其篩選龍湍１,２安保光１,２李新鵬１,２張維１,２李京琳１,２楊瑤華１,２曾翔１,２吳永忠１,２黃培勁１,２,?(１海南波蓮水稻基因科技有限公司,?？冢担罚埃保玻?２海南神農(nóng)大豐種業(yè)科技股份有限公司,?？冢担罚埃保玻??通訊聯(lián)系人,EＧmail:boＧlianrgt２０１５＠aliyun．com)Co
2、nstructionandScreeningofanIrradiationＧinducedMutantLibraryofindicaRice９３Ｇ１１LONGTuan１,２,ANBaoＧguang１,２,LIXinＧpeng１,２,ZHANGWei１,２,LIJingＧlin１,２,YANGYaoＧhua１,２,ZENGXiang１,２,WUYongＧzhong１,２,HUANGPeiＧjin１,２,?(１HainanBolianRiceGeneTechnologyCo．,Ltd．,Haikou５７０１２５,China;２
3、GrandAgriseedsTechnology,Inc．,Haikou５７０１２５,China;?Correspondingauthor,EＧmail:bolianrgt２０１５＠aliyunc．om)LONGTuan,ANBaoguang,LIXinpeng,etal．ConstructionandscreeningofanirradiationＧinducedmutantlibraryofindicarice９３Ｇ１１．ChinJRiceSci,２０１６,３０(１):４４Ｇ５２．Abstract:Mutantlibr
4、ariesarepowerfultoolsforplantgermplasmcreationandfunctionalanalysisofgenome．Using９３Ｇ１１(O．sativassp．indicacv．９３Ｇ１１),anelitevarietywithcompletegenomesequencesavailable,weestablishedanirradiationＧinducedmutantlibrarycontaining３６１７lines．Threehundredandeightmutantlines
5、wereobtainedfromM２andM３generationsandclassfiedinto１５typesofmutation,includingplantheight,tillernumber,plantarchitecture,inflorescencearchitecture,spikeletarchitecture,fertility,leafcolor,leafshape,headingdate,andbentazonresistance．Themutationfrequenciesrangedfrom０
6、．０３％to２．７４％．Usingreversegeneticsapproach,wefoundthatCYP７０４B２isthecausalgenefortwomalesterilemutantsandCYP８１A６isresponsiblefortwobentazonＧlethalmutations．Forthetwomalesterilemutants,nucleotidesubstitutionanddeletionweredetectedinthethirdandfourthexonsofCYP７０４B２,res
7、pectively．ForthetwobentazonＧlethalmutants,a７Ｇbpanda２３ＧbpdeletionweredetectedinthefirstandsecondexonsofCYP８１A６,respectively．GeneticstudiesrevealedthatwildＧtypeplantsandmutantplantssegregatedat３∶１ratio,suggestingthatthesemutanttraitswerecontrolledbysinglerecessivege
8、nes．Ourstudyshowedthatthismutantlibraryisausefulresourceforricegenomefunctionalanalysisandricegeneticimprovement．Keywords:indica;mutagenesisbyirradiatio
