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1、園藝學(xué)報(bào)2012,39(8):1559–1566http://www.ahs.ac.cnActaHorticulturaeSinicaE-mail:yuanyixuebao@126.com茉莉酸甲酯處理對(duì)葡萄果實(shí)NO和H2O2水平及植保素合成的影響1,2,*21112汪開(kāi)拓,鄭永華,唐文才,李廷君,張卿,尚海濤12(重慶三峽學(xué)院生命科學(xué)與工程學(xué)院,重慶404100;南京農(nóng)業(yè)大學(xué)食品科技學(xué)院,南京210095)-1摘要:以10μmol·L茉莉酸甲酯(Methyljasmonate,MeJA)熏蒸處理‘巨峰’葡萄果實(shí)6h,隨后轉(zhuǎn)入1
2、℃下貯藏28d。結(jié)果表明,MeJA處理顯著抑制了葡萄果實(shí)在貯藏期間腐爛率和失重率的上升,促進(jìn)內(nèi)源NO釋放量和H2O2含量在貯藏前期的上升,同時(shí)誘導(dǎo)植保素合成相關(guān)酶苯丙氨酸解氨酶(PAL)、肉桂酸–4–羥化酶(C4H)、對(duì)香豆酰–CoA連接酶(4-CL)和白藜蘆醇合成酶(RS)活性以及植保素白藜蘆醇和白藜蘆醇脫氫二聚體含量的上升。由此推測(cè),MeJA在葡萄果實(shí)細(xì)胞內(nèi)發(fā)揮了信號(hào)傳導(dǎo)作用,通過(guò)調(diào)控下游信號(hào)分子H2O2和NO的水平來(lái)提高植保素合成相關(guān)酶活性,從而促進(jìn)了植保素的積累,提高果實(shí)的抗病性,降低了其腐爛率。關(guān)鍵詞:葡萄;茉莉酸甲酯
3、;一氧化氮;過(guò)氧化氫;植保素中圖分類(lèi)號(hào):S663.1文獻(xiàn)標(biāo)識(shí)碼:A文章編號(hào):0513-353X(2012)08-1559-08EffectsofMethylJasmonateTreatmentonLevelsofNitricOxideandHydrogenPeroxideandPhytoalexinSynthesisinPostharvestGrapeBerries1,2,*2111WANGKai-tuo,ZHENGYong-hua,TANGWen-cai,LITing-jun,ZHANGQing,andSHANG2Hai-ta
4、o12(CollegeofLifeScienceandEngineering,ChongqingThreeGorgesUniversity,Chongqing404100,China;CollegeofFoodScienceandTechnology,NanjingAgriculturalUniversity,Nanjing210095,China)Abstract:Inordertorevealthemechanismsofmethyljasmonate(MeJA)inhibitingpostharvestdecay-1ing
5、rapeberries,‘Kyoho’grapeberrieswerepretreatedwith10μmol·LMeJAvaporfor6handthenstoredat1℃for28days.Thefruitdecayincidence,weightlossrate,levelsofendogenoussignalingmoleculesnitricoxide(NO)andhydrogenperoxide(H2O2),activitiesofphytoalexinsynthesisrelatedenzymesandindiv
6、idualphytoalexincompoundscontentsweredeterminedat7-dayintervalsduringthestorage.TheresultsexhibitedthattheMeJAtreatmentcouldsignificantlyinhibittheincreaseindecayincidenceandweightlossrate,promoteendogenousNOreleaseandH2O2contentduringtheearlystageofthestorage,andsim
7、ultaneouslyinducetheincreaseinactivitiesofphenylalanine-ammonia-lyase(PAL),cinnamate4-hydroxylase(C4H),4-coumaratecoenzymeAligase(4-CL)andresveratrolsynthase(RS)收稿日期:2012–04–06;修回日期:2012–07–20基金項(xiàng)目:重慶市自然科學(xué)基金項(xiàng)目(cstcjjA80018);重慶市教委科學(xué)技術(shù)研究項(xiàng)目(KJ111109);重慶市高校創(chuàng)新團(tuán)隊(duì)建設(shè)計(jì)劃項(xiàng)目(2010
8、40);國(guó)家自然科學(xué)基金項(xiàng)目(31172003)*E-mail:wangkaituo83@gmail.com1560園藝學(xué)報(bào)39卷associatedwithphytoalexinsynthesisandthecontentsofphytoalexinsincl