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1、茶葉科學(xué)2008，28（4）：301~308JournalofTeaScience葉綠素儀（SPAD）在茶樹(shù)氮素營(yíng)養(yǎng)診斷中的適用性研究1,2222*1*楊亦揚(yáng)，馬立鋒，石元值，阮建云，黎星輝（1.南京農(nóng)業(yè)大學(xué)茶葉研究所，江蘇南京210095；2.農(nóng)業(yè)部茶葉化學(xué)工程重點(diǎn)實(shí)驗(yàn)室，中國(guó)農(nóng)業(yè)科學(xué)院茶葉研究所，浙江杭州，310008）摘要：測(cè)定葉片氮素含量是診斷茶樹(shù)氮素狀況的一項(xiàng)重要方法。本文利用盆栽試驗(yàn)，通過(guò)不同的供氮水平，研究了利用葉綠素儀（SPAD-502）進(jìn)行茶樹(shù)氮素營(yíng)養(yǎng)快速診斷的適用性。結(jié)果表明，茶樹(shù)新梢產(chǎn)量與氮素用量、成熟葉氮素含
2、量之間呈線性加平臺(tái)的反應(yīng)關(guān)系，成熟葉的SPAD值與其全氮含量呈顯著線性正相關(guān)，并與茶樹(shù)產(chǎn)量存在著比較明顯的關(guān)系拐點(diǎn)，初步顯示SPAD可以較好地表征茶樹(shù)的氮素營(yíng)養(yǎng)狀況，有可能作為快速診斷方法用于指導(dǎo)茶樹(shù)施肥，但是還需要通過(guò)大田試驗(yàn)進(jìn)行進(jìn)一步驗(yàn)證。本文還探討了測(cè)定位點(diǎn)、葉位、天氣狀況、田間原位或離體測(cè)定方式、表面清洗以及樣本量等因素對(duì)SPAD測(cè)試精度的影響。關(guān)鍵詞：茶樹(shù)；葉綠素儀；氮素營(yíng)養(yǎng)；影響因素中圖分類號(hào)：S571.1；S143.1文獻(xiàn)標(biāo)識(shí)碼：A文章編號(hào)：1000-369X（2008）04-301-08EvaluationofNit
3、rogenStatusinTeaPlantsbySPAD1,2222*1*YANGYi-yang,MALi-feng,SHIYuan-zhi,RUANJian-yun,LIXing-hui（1.TeaResearchInstitute,NanjingAgricultureUniversity,Nanjing210095,China;2.KeyLaboratoryofTeaChemicalEngineering,theMinistryofAgricultureandTeaResearchInstituteofChineseAcadem
4、yofAgricultureSciences,Hangzhou310008,China）Abstract:DeterminationofnitrogenconcentrationsintissuesisanimportantdiagnosticmeansforNstatusofplants.ApotexperimentwithteaplantsappliedwithdifferentamountsofNfertilizerwascarriedouttoevaluatetheusefulnessofSPAD-502asadiagnos
5、tictool.TheresultsshowedthattheyieldofyoungshootsandNconcentrationofmatureleavesrespondedtoNapplicationrateswithapatternofaninitiallinearincreasebeforeaplatformwasreached.TherewasasignificantlypositivelinearrelationshipbetweentheSPADreadingandtotalNconcentrationofmatur
6、eleaves.ThesimilarpatternofaninitiallinearincreasefollowedbyaplatformwasobservedfortherelationshipbetweenSPADreadingandtheyield.TheseresultssuggestedthattheNstatusofteaplantsmaybecharacterizedbythemeasurementofSPADreadingofmatureleavesandtheSPADisapotentiallyusefulrapi
7、ddiagnostictoolinNfertilizationrecommendation.However,thisneedsfurthervalidationfromfieldexperiments.ThosefactorswhichmayinfluencetheprecisionofSPADmeasurement,suchasthepointoftestonaleaf,selectionofleavesfromtwig(position),weathercondition,insituorinvitroanalysis,surf
8、acecleaningandthenumberofleavesforacompositesamplewerealsoinvestigated.Keywords:CamelliasinensisL.,chlorophyllmeter,n
