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1、窗體頂端Select1stenzyme:????Select2ndenzyme:????窗體底端EnzymeCat#TempSuppliedNEBufferSupplements%ActivityinNEBufferBSASAM1234BamHI*R013637°CNEBuffer3YesNo75100100100KpnI*R014237°CNEBuffer1YesNo10075050DoubleDigestRecommendation(s)forBamHI+KpnI:§Doubledigestisnotrecommended.A
2、sequentialdigestisrequired,usingeachenzymeinitssuppliedNEBuffer.Note:Theaboverecommendationisbasedontheexperimentalresults.PleasecheckSuggestedNEBuffersforDoubleDigestion.*??BamHIhasaHighFidelityversionBamHI-HF(R3136)????KpnIhasaHighFidelityversionKpnI-HF(R3142)????Hi
3、ghFidelity(HF)RestrictionEnzymeshavebeenengineeredforreducedstaractivity????andhave100%activityinbuffer4whichmaysimplifyyourdoubledigest.DoubleDigestsDigestingaDNAsubstratewithtworestrictionendonucleasessimultaneously(doubledigestion)isacommontimesavingprocedure.Selec
4、tingthebestNEBuffertoprovidereactionconditionsthatoptimizeenzymeactivityaswellasavoidstaractivityassociatedwithsomeenzymesisanimportantconsideration.EachenzymeissuppliedwithitsoptimalNEBuffertoensure100%activity.NEBuffercompositionsarelistedonbufferpagesandonthedataca
5、rdsentwitheachenzyme.TheActivityChartforRestrictionEnzymesratesthepercentageactivityofeachrestrictionendonucleaseinthefourstandardNEBuffersandNEBufferEcoRI.PleaseuseNEBDoubleDigestFindertoperformadoubledigestcalculation.SuggestedNEBuffersforDoubleDigestionEnzyme?AatII
6、AvrIIBamHIBglIIBsgIEagIEcoRIEcoRVHindIIIKpnIMseINcoINdeINheINotIPstIPvuISacISacIISalISmaISpeISphIXbaIXhoIXmaINEBuffer443343EcoRI3214342333143442444AatII4--4seqseq4seqseq4444444seq4seq44seq444444AvrII44--3243EcoRI2214442332143442444BamHI3seq3--333EcoRI3seqseq333seq333s
7、eqseq3seqseq333seqBglII3seq23--33EcoRI3222332333223seq22232BsgI44433--seqseq42seq4444333443444444EagI3seq333seq--EcoRI3seqseq333seq333seqseq3seqseq333seqEcoRIEcoRIseqEcoRIEcoRIEcoRIseqEcoRI--EcoRIseq1EcoRIEcoRIEcoRI1EcoRIEcoRIEcoRI1EcoRIEcoRIseqEcoRIEcoRIseqEcoRIseqEc
8、oRV3423343EcoRI--222322333223422234HindIII242seq22seqseq2--2222222222seq42222seqKpnI141seq2seqseq122--111121214seqseq11214Ms
