CNQX對(duì)乙醇、乙醛調(diào)控大腦皮質(zhì)神經(jīng)元神經(jīng)甾體水平的影響

《CNQX對(duì)乙醇、乙醛調(diào)控大腦皮質(zhì)神經(jīng)元神經(jīng)甾體水平的影響》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫(kù)。

1、萬(wàn)方數(shù)據(jù)史堡超絲匡堂參圭一2--010生墨旦箜芝鲞一笪8勞JChinJNeuromed,August2010,V01．9,No．8CNQX對(duì)乙醇、乙醛調(diào)控大腦皮質(zhì)神經(jīng)元神經(jīng)甾體水平的影響張翠欣侯艷寧劉紅衛(wèi)張彤【摘要】目的研究乙醇、乙醛對(duì)原代培養(yǎng)大鼠大腦皮質(zhì)神經(jīng)元神經(jīng)甾體水平的影響以及與AMPA受體調(diào)控的相關(guān)性。方法應(yīng)用AMPA受體拮抗劑CNQX阻斷AMPA受體．用50mlnol／L乙醇或25mmol／L乙醛處理神經(jīng)元，收集細(xì)胞培養(yǎng)上清，采用高效液相色譜質(zhì)譜法分離測(cè)定游離型神經(jīng)甾體孕烯醇酮(PREG)、脫氫表雄酮(DHEA)、別孕烯醇酮(AP)及結(jié)合型神經(jīng)甾體脫

2、氫表雄酮硫酸酯(DHEAS)、孕烯醇酮硫酸酯(PREGS)的含量。觀察CNQX對(duì)乙醇、乙醛調(diào)控原代培養(yǎng)神經(jīng)元神經(jīng)甾體水平的影響。結(jié)果乙醇可明顯升高神經(jīng)元PREG、AP和PREGS的水平，與PBS對(duì)照組比較差異有統(tǒng)計(jì)學(xué)意義(風(fēng)0．05)；乙醛可明顯升高神經(jīng)元PREG、DHEA、PREGS和DHEAS的水平，與PBS對(duì)照組比較差異有統(tǒng)計(jì)學(xué)意義職O．05)。CNQX反轉(zhuǎn)了乙醇升高PREG、PREGS水平的作用以及乙醛升高PREG、DHEAS水平的作用。卻使AP水平明娃升高。組間比較差異有統(tǒng)計(jì)學(xué)意義(氏0．05)。結(jié)論乙醇、乙醛對(duì)大腦皮質(zhì)神經(jīng)元神經(jīng)甾體PREG、DHE

3、A、AP、PREGS和DHEAS的影響各不相同，CNQX反轉(zhuǎn)了乙醇上調(diào)PREG、PREGS水平的作用以及乙醛上調(diào)PREG、DHEAS水平的作用?！娟P(guān)鍵詞】CNQX；乙醇；乙醛；神經(jīng)元；神經(jīng)甾體【中圖分類號(hào)】R917．101【文獻(xiàn)標(biāo)志碼】A【文章編號(hào)】1671—8925(2010)一08—777—05CNQXaffectingregulationabilitiesofethanolandaldehydeOillevelsofneurosteroidincorticalne呱omZHANGCui-xin*,HOUYan-ning,LIUHong--wei*,ZHA

4、NGTon礦．*DepartmentofPharmacy,死刪HospitalofHebeiMedicalUniversity,ShO'iazhuang050051．China【Abstract】ObjectiveToinvestigatetheeffectsofethanolandaldehydeonthelevelsofneurosteroidinthecorticalneuronsofprimary-culturedmtsandexploretheirrelationswinltheregulationofAMPAreceptor．MethodsCNQX

5、(theAMPAreceptorantagonist)wasusedtoblocktheAMPAreceptor,andthen，50mmol／Lethanolor25mmol／Laldehydewasemployedtotreattheneurons；supernatewascollected．HPLC·MS／APCIwasemployedtoanalyzeanddeterminetheconcentrationsofunconjugatedneurosteroids(pregnenolone[PREG]，dehydroepiandrosterone[DHE

6、A]，allopregnanolone【AP】)andconjugatedneurosteroids(dehydroepiandrosterone【DHEAS]，pregnenolonesulphate【PREGS])inmediafromprimaryculturalcerebralcortexnellrons．TheeffectsofCNQXontheregulationabilitiesofethanolandaldehyde011levelsofneurosteroidsinprimary-culturedcerebmlcortexneuronswer

7、edetected．ResultsThelevelsofPREG，APandPREGSintheethanoltreatmentgroupweresignificantlyincreasedascomparedwiththoseinthePBStreatmentgroup(P<0．05)；thelevelsofPREG，DHEA，PREGSandDHEASinthealdehydetreatmentgroupweresignificantlyincmasedascompared誦mthoseinthePBStreatmentgroup(P

8、reversedtheeffectof