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1、雷帕霉素對大鼠腦缺血再灌注損傷的保護(hù)效應(yīng)研究王麗君吳登艷胡安春楊晨景玉宏*1?蘭州大學(xué)基礎(chǔ)醫(yī)學(xué)院（甘肅蘭州730000）2?蘭州大學(xué)基礎(chǔ)醫(yī)學(xué)院神經(jīng)科學(xué)研究所（廿肅蘭州730000）基金資助：蘭州大學(xué)本科生創(chuàng)新創(chuàng)業(yè)行動(dòng)計(jì)劃資助*通訊作者簡介：景玉宏，男，I専士，副教授，蘭州大學(xué)基礎(chǔ)醫(yī)學(xué)院神經(jīng)科學(xué)研究所,Email:jingyh@lzu.edu.cn【摘要】目的：通過采用雙側(cè)頸總動(dòng)脈阻斷的方法制作腦缺血再灌注動(dòng)物模型，研究雷帕霉素對大鼠腦缺血再灌注損傷是否冇保護(hù)效應(yīng)。方法：用雙側(cè)頸總動(dòng)脈阻斷結(jié)扎法制作缺血模型，缺血前側(cè)腦室給藥，于缺血20min后再灌
2、注，24h后心臟灌注固定，取腦做連續(xù)冠狀冰凍切片，光鏡下觀察拍照，無偏差計(jì)數(shù)法計(jì)算細(xì)胞密度。結(jié)果：統(tǒng)計(jì)結(jié)呆顯示假手術(shù)組和缺血組的分子層細(xì)胞較少，而錐體細(xì)胞層的細(xì)胞較多；從前額皮層和額葉來看，顓葉的細(xì)胞密度較低，而前額皮層的細(xì)胞密度較高；給予Rapa,可以看出，缺血前12h給藥組的細(xì)胞數(shù)較多，而缺血前30min給藥組的細(xì)胞較少。由此可得損傷主要在潁葉的分子層；缺血前12h給雷帕霉索的效果優(yōu)于缺血前30min給雷帕霉索。結(jié)論：雷帕霉索對大鼠腦缺血再灌注損傷有保護(hù)效應(yīng)?！娟P(guān)鍵詞】雷帕霉素；腦缺血；再灌注損傷；保護(hù)Toinvestigatetheprot
3、ectiveeffectofRapamycinoncerebralischemia-reperfusioninjuryinrats.WANGLijun,WUDengyan,HUAnchun,YANGCheng,JINGYuhong.1.SchoolofBasicMedicalSciences,LanzhouUniversity,Lanzhou730000,Gansu,China?2.InstituteofNeuroscience,LabofNeurodegenerativedisease,LanzhouUniversity,Lanzhou7300
4、00,Gansu,China.Fundedby:UniversityStudents'InnovationandVenturePlanofLanzhouUniversity.^Correspondingauthor:JINGYuhong,doctor,associateprofessor,InstituteofNeuroscience,LabofNeurodegenerativedisease,LanzhouUniversity,Email:jingyh@lzu?edu.cn（Abstract）Objective:throughtheuseofb
5、ilateralcarotidarteryocclusionmethodproducedanimalmodelsofcerebralischemiatostudytherapamycinoncerebralischemia-reperfusioninjuryhasprotectiveeffects.Methods:Withbilateralcarotidarteryocclusionmodelofischemiabyligation,intracerebroventricularadministrationbeforeischemia,reper
6、fusionafter20minischemia,after24h,perfusionandfixation,andthebraintodoconsecutivecoronalfrozensectionsobservedunderlightmicroscopepictures,unbiasedcountingmethodbasedoncelldensity.Results:Thestatisticsindicatedthat,comparedwiththepyramidalcelllayer,thecellsofthemolecularlayer
7、intheshamgroupandischemiagroupareless;fromthepointofview,prefrontalcortexandtemporallobe,thecelldensityofthetemporallobeislower,whilethecelldensityofprefrontalcortexismuchhigher;givingrapamycin,wecanseethat12hbeforeischemiahasmorecellsthan30minbeforeischemia?Inaword,theinjury
8、ismainlyinthemolecularlayerofthetemporallobe;andcomparedwithdosingat
