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1、※生物工程食品科學(xué)2015,Vol.36,No.21123耐酸耐膽鹽乳酸菌的鑒定及篩選122211,*，韓曉日1李利，孔麗，張寧，趙偉超，肖亦農(nóng)，李炳學(xué)（1.沈陽農(nóng)業(yè)大學(xué)土地與環(huán)境學(xué)院，土肥資源高效利用國家工程實(shí)驗(yàn)室，遼寧沈陽110866；2.沈陽農(nóng)業(yè)大學(xué)生物科學(xué)技術(shù)學(xué)院，遼寧沈陽110866）摘?要：從自然發(fā)酵的酸奶中分離出2株細(xì)菌，經(jīng)16SrdNA分子鑒定為LactobacillusplantarumSN1和LactobacillusrhamnosusSN6，并對其生長曲線、產(chǎn)酸速率、耐酸耐膽鹽能力進(jìn)行了研究。L.plantarumSN1和L.rhamnosusSN6在
2、2h后進(jìn)入對數(shù)期，16h后達(dá)到穩(wěn)定期，其OD600nm值分別為8.47、7.43。L.plantarumSN1和L.rhamnosusSN6的產(chǎn)酸速率較快，ph值在8h后就降到了4.2以下，48h后降到3.3左右。L.plantarumSN1和L.rhamnosusSN6在ph4的培養(yǎng)基培養(yǎng)16h后，其相對OD600nm值分別為49.29%、47.14%，具有較強(qiáng)的耐酸能力。L.plantarumSN1和L.rhamnosusSN6在0.3g/l膽鹽質(zhì)量濃度下培養(yǎng)16h后，相對OD600nm值分別為57.7%、69.48%；在0.6g/l膽鹽質(zhì)量濃度下的相對OD600nm值
3、分別為48.22%、29.56%，具有較強(qiáng)的耐膽鹽能力。結(jié)果表明：L.plantarumSN1和L.rhamnosusSN6是生長性能好、產(chǎn)酸能力強(qiáng)、耐酸耐膽鹽的益生菌株。關(guān)鍵詞：乳酸菌；耐酸耐膽鹽；16SrdNA序列分析Identi?cationandSelectionofLacticAcidBacteriaResistanttoAcidandBileSalt122211,*,HANXiaori1LILi,KONGLi,ZHANGNing,ZHAOWeichao,XIAOYinong,LIBingxue(1.NationalEngineeringLaboratoryfor
4、Ef?cientUtilizationofSoilandFertilizerResources,CollegeofLandandEnvironment,ShenyangAgriculturalUniversity,Shenyang110866,China;2.CollegeofBiologicalScienceandTechnology,ShenyangAgriculturalUniversity,Shenyang110866,China)Abstract:Twostrainsoflacticacidbacteriawereisolatedfromnaturallyferm
5、entedyogurt.The16SrdNAsequenceanalysisindicatedthatthetwostrainswereLactobacillusplantarumSN1andLactobacillusrhamnosusSN6.Thesestrainswerestudiedforgrowthcurve,acidproductionrate,acidandbilesalttolerance.Bothofthemreachedalogarithmicgrowthphaseafter2handastableperiodafter16hwithod600nmvalu
6、esof8.47and7.43,respectively.ThepHvaluedroppedto4.2at8hand3.3at48h.L.plantarumSN1andL.rhamnosusSN6hadstrongresistancetoacid,andtherelativeod600nmvaluewas49.29%and47.14%atpH4.0after16hofculture,respectively.Afterbeingculturedfor16hatabilesaltconcentrationof0.3g/l,therelativeod600nmvaluesofL
7、.plantarumSN1andL.rhamnosusSN6were57.7%and69.48%,respectively,comparedto48.22%and29.56%whentheconcentrationofbilesaltwas0.6g/l.TheseresultsshowthatbothL.plantarumSN1andL.rhamnosusSN6areprobioticstrainswithstronggrowthperformance,acidproductionandtolerancetoaci
