水稻恢復(fù)系8821花粉育性中性基因的鑒定

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1、水稻恢復(fù)系8821花粉育性中性基因的鑒定董慶磊，陳鵬飛，王思哲，吳錦文，劉向東（華南農(nóng)業(yè)大學(xué)亞熱帶農(nóng)業(yè)生物資源保護(hù)與利用國家重點(diǎn)實(shí)驗(yàn)室，廣東廣州510642）摘耍：制粳亞種間雜種F1具有強(qiáng)人的生物學(xué)優(yōu)勢，然而F1育性普遍偏低影響其產(chǎn)量優(yōu)勢發(fā)揮?；ǚ鄄挥且鹩跃s種F1育性偏低的最重要原WZ-,主要山S-a.S-b和S-c等至少6個(gè)基因座位的互作引起，而各座位上的屮性基因有望克服其不育性，所以挖掘和利川花粉育性中性基因具有重耍意義。本文利用水稻恢復(fù)系品種8821分別與粳稻臺中65及其攜帶有花粉不育基因的近等基因系雜交組呢F1,對其F1的花

2、粉育性進(jìn)行觀察，結(jié)果發(fā)現(xiàn)S?a、S-b互作的各雜種F1花粉育性與無互作的雜種F1比較，彼此差異不顯著;進(jìn)一步利用分子標(biāo)記檢測F2群體中的3種基因型分離比例，結(jié)果符合孟徳爾分離比例（1:2:1）,說明8821在S-a和S-b座位上同時(shí)攜帶有釉粳雜種F1花粉育性中性基因，該材料有望克服大部分的釉粳雜種不育性，值得進(jìn)一步深入研究。關(guān)鍵詞：水稻；花粉育性；中性基因；恢復(fù)系中圖分類號:S326IdentiHcationontheneutralgenesforpollen-sterilityinricerestorerline8821DONGQing

3、lei,CHENPengfei,WANGSizhe,WUJinwen,LIUXiangdong(StateKeyL

4、seofitsgiganticfeatures.However,partialsterilityinthehybridshasbecomeamajorobstacleforusingtheheterosisincommercialproduction.Pollensterilityisoneofthemostimportantreasontocausethelowfertilityofindica-japonicahybridsFLAtleastSixlocicontrollingthepollensterilityofintcr-sub

5、spccifichybridshavebeenidentifiedandwasfoundneutralallelesateachlocusarcpotentialtoovercometheFlpollensterilityassociatedwiththelocusfrompreviousstudies?Therefore,exploitationandutilizationofneutralgenesareofsignificantimportance.Taichung65anditspollen-sterilitynear-isoge

6、niclineswereusedastestparentsfordevelopingtest-crossesandtheirFlpollenfertilitywasexaminedinthepresentstudy.TheresultsshowedthatthepollenfertilitywerenotsignificantdifferencesbetweenFlwithinteractioninS-aandS-bloeouandtheFlwithnointeractioninthesamelocous?Theplantswiththr

7、eedifferentgenotypesinF2werecheckedbyusingmolecularmarkersandwasdisplayedthatsegregationofthe3genotypesfollowedtheexpectedMendelianratio(1:2:1)intheF2populations.Evidentially,8821carriedtheneutralgenesatS-aandS-blocus,whicharepotentialtoovercomethemostlypollen-sterilityof

8、hybridsbetweenindicaandjaponicaandneedopentostudy.Keywords:rice;pollenfertility;neutralgenes;ric