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1、JAN20,2009,36(1):20-24?2009byInstituteofMicrobiology,CAS微或物修通報(bào)Microbiologytongbao@im.ac.cn罰究報(bào)告海洋蛭弧菌的分離鑒定及其對(duì)副溶血弧菌的作用儲(chǔ)衛(wèi)華I*朱衛(wèi)'康春濤2（1.中國商科大學(xué)生命科學(xué)與技術(shù)學(xué)院江蘇南京210009）（2.江蘇星海動(dòng)物藥業(yè)科技有限公司江蘇東臺(tái)224233）摘要：蛭弧菌廣泛存在于自然水體，具有噬菌的特性，對(duì)水體中細(xì)菌數(shù)量控制具調(diào)節(jié)作用。以副溶血弧菌為宿主菌，利用雙層瓊脂法，從海洋水體中分離出15株具有噬菌作用的細(xì)菌，對(duì)形成噬菌斑能力最強(qiáng)的1株
2、菌株進(jìn)行特異性16SrDNA擴(kuò)增，確認(rèn)為蛭弧茵，命名為Bd-MLBd-Ml對(duì)大多數(shù)海水養(yǎng)殖動(dòng)物病原菌有裂解作用，裂解率在90%(20/22)以上，模擬水環(huán)境實(shí)驗(yàn)發(fā)現(xiàn)，蛭弧菌對(duì)副溶血弧茵有較強(qiáng)的裂解和凈化作用，102h內(nèi)能使副溶血弧菌從3.0xl08CFU/mL下降到8.7xl03CFU/mL。動(dòng)物實(shí)驗(yàn)表明蛭弧菌能有效預(yù)防對(duì)蝦弧菌病的發(fā)生，表明蛭弧菌有望成為水產(chǎn)動(dòng)物疾病防治的一種有效的生物制劑。關(guān)鍵詞：副溶血弧菌，蛭弧茵，對(duì)蝦，生物防治Isolation,IdentificationofMarineBdellovibriosandItsEffecton
3、VibrioparahaemolyticusCHUWei-Hua1*ZHUWei1KANGChun-Tao2(1.DepartmentofMicrobiology.SchoolofLifeScience&Technology.ChinaPharmaceuticalUniversity,Nanjing,Jiangsu210009,China)(2.JiangsuXinghaiVeterinaiyPharmaceuticalCo.、Ltd“Dongtai,Jiangsu224233,China)Abstract:Bdellovibriosarcoblig
4、atepredatorybacteria,widelydistributeinnatureandregulatethecountsofbacteriuminwater.FifteenstrainsofpredatorybacteriawereisolatedfrommarineenvironmentusingVibrioparahaemolyticusDX-1ashostandoneofthemformedlargeplaquesdesignatedasBd-M1.ThestrainwasconfirmedbyBdellovibriospecies-
5、specific16SrDNAfragmentamplifiedusingPCRmethod.Determinationofhostrangeonspeciesoffishpathogenicbacteria(Vibrioparahaemolyticus,Vibrioalginolyticus^Vibrioan^uillarmn.Vibrioanguillarum.VibriovulnificusandEdwardsiellatarta)indicatedthatBd-Mlwasabletoparasitize20strainsoutofatotal
6、of22tested.Co-cultureexperimentshownthatBd-MlhavepurificationfunctiononVibrioparahaemolyticus,thenumberofVparahaemolyticusdecreasedfrom3.Ox108CFU/mLto8.7x1()'CFU/mLduring102h.ImmersionPenaeusvannatneiinwateratdifferentconcentrationofBd-Mlwasusedinprotectionagainstexperimentalin
7、fectionwithV.parahaemolyticusDX-1infection.ResultsshownthatthemortalityofgroupsbathedwithBd-MlwaslowerthanthatgroupwithoutBd-M1.Theseresultssuggestthatitmaybepossibletousebdellovibriotocontrolfishdisease?Keywords:Vibrioparahaemolyticus,Bdellovibrios.Penaeusvannamei,Bio-therapy水
8、通訊作者：Tel:86-25-83271323;chuweihua2002@yahoo.com.cn收稿日期
