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1、以Fe(III)為電子受體聚磷菌篩選、鑒定及聚磷特性作者簡(jiǎn)介：任麗平(1971),女，副教授，博士生，主要從事資源微生物和水污染控制研究，(Email)xhrlph@163.com0張智(通信作者)，男，教授，博士生導(dǎo)師，(Email)zhangzhicq@126.com0摘要：結(jié)合平板分離法、poly-P和PHB染色以及檸檬酸鐵還原實(shí)驗(yàn)，從污水處理廠活性污泥中分離獲得一株以Fe(III)為電子受體厭氧聚磷的聚磷菌株AP3,其16SrDNA與PseudomonasmosseliiATCCBAA-99的同源性為99%。菌株AP3于檸檬酸鐵液體培養(yǎng)基和無(wú)鐵液體培養(yǎng)基中厭氧避光
2、、27°C靜置培養(yǎng)，在含有Fe(III)的培養(yǎng)基中比在無(wú)Fe(III)的培養(yǎng)基中生長(zhǎng)好；菌株AP3在無(wú)Fe(III)的培養(yǎng)基中，表現(xiàn)出典型的厭氧釋磷特征，釋磷量為3.68mg?L-1；在含有Fe(III)的培養(yǎng)基中，先厭氧釋磷，釋磷量為6.62mg?L-1,10h之后開(kāi)始厭氧聚磷,聚磷量為5.89mg?L-lo菌株AP3能以Fe(III)為電子受體,具有厭氧聚磷特征。關(guān)鍵詞：聚磷菌；篩選；鑒定；氧化鐵；電子受體中圖分類(lèi)號(hào)：X172文獻(xiàn)標(biāo)志碼：A文章編號(hào)：16744764(2012)03014605ScreeningandIdentificationofPhosphateA
3、ccumulatingBacteriawithFe(III)asElectronicAcceptoranditsEffectsonPhosphorusAccumulatingCharacteristicsRENLipingl,2,ZHANGZhil,TANGYun2(1.CollegeofUrbanConstruetionandEnvironmentalEngineering,ChongqingUniversity,Chongqing400045,P.R.China；2.CollegeofBiologicalSciences,ChinaWestNormalUniversi
4、ty,Nanchong637002,P.R.China)Abstract：AphosphorusaccumulationorganismstrainAP3withFe(III)aselectronicacceptorwasscreenedfromactivatedsludgethroughsuchprocessesasdilutionplatemethod,polyP,PHBstainingandferriccitratereduction.Its16SrDNAisidenticaltothatofPseudomonasmosseliispATCCBAA99at99%le
5、vel.BycomparingtheAP3beingculturedinferriccitratefluidmediumandtheoneinnonferricfluidmediumat27°C,itwasfoundthatferriccitratefluidmediumwasbeneficialfortheAP3?AP3culturedinnonferricfluidmediumshowedsignificantanaerobicandphosphorusreleasingcharacteristic,thephosphorusreleasingamountofwhic
6、hwas3.68mg?L—l.AP3culturedinfeiricfluidmediumwasalsocharacterizedbyanaerobicandphosphorusreleasingpropertyatthefirststage,thephosphorusreleasingamountofwhichwas662mg?LT,whichwashigherthantheformer；AndatthesecondstagewhichstartedfromthelOh,itwascharacterizedbyanaerobicandphosphorusaccumula
7、tingproperty,thephosphorusaccumulatingamountofwhichwas5.89mg?L~1.Fe(III)istheelectronacceptorofAP3whichshowstypicalanaerobicandphosphorusabsorbingproperty.Keywords:phosphorusaccumulationorganism；screening；identification；ironoxides；electronicacceptor現(xiàn)代污水生物強(qiáng)化除磷法是通過(guò)聚磷菌
