FoxP3基因在胃癌患者Treg細胞中的表達及其對Treg細胞的調(diào)節(jié)作用

《FoxP3基因在胃癌患者Treg細胞中的表達及其對Treg細胞的調(diào)節(jié)作用》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫。

1、萬方數(shù)據(jù)156陜西醫(yī)學雜志2009年2月第38卷第2期FoxP3基因在胃癌患者Treg細胞中的表達及其對Treg細胞的調(diào)節(jié)作用河北工程大學附屬醫(yī)院普外三科(邯鄲056029)趙素斌李磊白凈付澤嫻摘要目的：研究Foxp3基因在CD4+、CD25+調(diào)節(jié)性T細胞(Treg細胞)中的表達水平及其對Treg細胞的調(diào)節(jié)作用。方法：應用流式細胞術(shù)(FACS)測定實驗組和對照組患者術(shù)前外周血中T淋巴細胞及Treg細胞含量的改變；采用RT—PCR技術(shù)檢測FoxP3基因在胃癌患者外周血Treg細胞中的表達與對照組的差異。結(jié)果：胃癌患者外周血中T淋巴細胞(CD3+T)及抑制殺傷性T細胞(CD8

2、+T)總數(shù)，與健康對照組相比無明顯統(tǒng)計學差異(P>o．05)；Treg細胞含量顯著高于健康對照組(P

3、rcinomapatientsAffiliatedHospitalofHebeiEngineerUniversity(Handan056029)ZhaoSubinLiLeiBaiJingetalABSTRACTObjective：ToinvestigateexpressionofFoxP3geneinTregcellsandthepossiblefunctioninTregcellsinregulatinganti-cancerresponseinthegastriccarcinomapatients．Methods：Byusingflowcytometry，thelev

4、elsofCD3+Tcells，CD8+Tcells，andTregcellsinperipheralbloodofpreoperativepatientswithgastriccancerweredetectedrespectively，andtheresultswereanalyzedstatisticallyincomparisonwiththatofthehealthyvolunteers；RT—PCRtechniqueswereusedtodetecttheexpressionofFoxP3geneintheseTregcells，andtheresultswe

5、reanalyzedstatisticallyincomparisonwiththatofthehealthyvolunteers．Results：ThepercentagesoftotalTlymphocytes(CD3+T)andinhibitingkillerTcells(CD8+T)inperipheralbloodofthepreoperativepatientswithgastriccancerwerefoundtobenodifferentstatisticallyincomparisonwiththatofhealthyvolunteers(P>0．05)

6、；thepercentageofTregintotalCD4+Tcellsinpatientswithgastriccancerincreasedsignificantlycomparedwiththatinvolunteers(P

7、ressionofTregcellsindicatesthatFoxP3genesmayplayapivotalroleinregulatingthefunctionofTregcellsinself—tumorimmunityofthepatientswithgastriccancer．KEYWoRDSStomachneoplasmsGeneexpressionregulation@Tregceils@FoxP3gene前期研究表明[1]：胃癌患者外周血中Treg細胞的數(shù)量較正常人明顯增高，可能在機體對腫瘤的自身免疫中發(fā)揮重