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1、基質(zhì)金屬蛋白酶8在食管鱗癌中的表達及其臨床意義王冬梅1'［郭洪1,陳雪丹1,官興穎1,劉丹1，白云(400038重慶，第三軍醫(yī)大學基礎(chǔ)部遺傳教研室'；430010武漢，解放軍161醫(yī)院檢驗科3［摘要］目的探討食管鱗狀細胞癌中基質(zhì)金屬蛋白酶8(MMP-8)的表達及共與腫瘤浸潤、轉(zhuǎn)移的關(guān)系。方法實時熒光定量I忙R(Real-timePCR)方濃檢測67例食管鱗癌組織和27例遠癌組織'PMMP-8mRNA的表達；Westernblot法檢測英中15例食管鱗癌組織及其配對的15例遠癌組織中MMP-8蛋白的表達，統(tǒng)計分析MMP-8表達水平與食管鱗
2、癌的臨床分期以及浸潤轉(zhuǎn)移的關(guān)系。結(jié)果(1)刪卩-8在食管鱗癌組織和遠癌組織屮均有不同程度的表達，在癌組織的表達水平顯著低于遠癌組織5RNA表達水平E0.003,蛋白表達水平E0.031)。(2)T1期、T2期、T3期以及T4期食管鱗癌組織tMMP-8mRA表達存在顯著差異(1M).002),徘11)-8表達隨著腫瘤浸潤程度的加深而升高；MM—8蛋白表達量在T1?T4期中也呈增加的趨勢J3)M.MP-8mRXA和蛋白表達水平與食管鱗癌的淋巴結(jié)轉(zhuǎn)移無顯著關(guān)聯(lián)。結(jié)論食管鱗癌組織中MMP-8的表達較遠癌組織降低，其表達水平又與食管鱗癌的浸潤程
3、度正相關(guān)，可能反映了MMP-8在食管鱗癌發(fā)生、發(fā)展過程中的不同作用機制。［關(guān)鍵詞］食管鱗狀細胞癌；基質(zhì)金屬蛋白酶8；浸潤；轉(zhuǎn)移；表達［中圖法分類號］R394.3;R730.2;R735.1［文獻標志碼］AExpressionofMatrixMetalloproteinase8inEsophagealSquamousCellCarcinomaWangDongmei12,GuoHong1,ChenXuedan1,GuanXingying1,LiuDan1,BaiYun1(DepartmentofMedicalGenetics,College
4、ofBasicMedicalScience,ThirdMilitaryMedicalUniversity1,Chongqing,40003&DepartmentofLaboratoryMedical,161HospitalofPLA2,Wuhan430010)ExpressionandClinicalSignificanceofMatrixMetalloproteinase8inEsophagealSquamousCellCarcinoma［Abstract］ObjectiveMatrixMetalloproteinase8(MMP-8
5、)playsmultiplerolesindifferentcancersordifferentstageincancerdevelopment.However,thereisnoreportaboutMMP-8expressionandroleinesophagealsquamouscellcarcinoma(ESCC)sofbr.TheaimofthisstudyistodeterminetheexpressionofMMP-8inESCCandexplorethecorrelationsbetweentheexpressionof
6、MMP-8andinvasionandmetastasisofESCC.MethodsWedetectedMMP-8mRNAexpressionin67ESCCtissuesand27normaltissueswithReal-timePCRandalsodetecteditsproteinexpressionin15ESCCtissuesand15matchednormaltissueswithWesternblot,thenexploredtheassociationofMMP-8expressionwithclinicalchar
7、acteristicsincludinginvasionandmetastasis.ResultsThemRNAandproteinexpressionlevelsofMMP-8weremarkedlylowerinESCCtissuesthanthatintheadjacentnormaltissues(mRNA:P=0.003,protein:P=0.031).Inaddition,MMP-8mRNAexpressivelevelwaspositivelycorrelatedwithtumorinvasiondepth(P=0.00
8、2).TherewasalsoatrendencythatMMP-8proteinexpressionincreasedastheelevateddepthoftumorinvasion.However,t
