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1、FieldIsolatesofTransmissibleGastroenteritisVirusDifferattheMolecularLevelfromtheMillerandPurdueVirulentandAttenuatedStrainsandfromPorcineRespiratoryCoronavirusesHyukMooKWON*,LindaJ.SAIFandDaralJ.JACKWOOD**FoodAnimalHealthResearchProgram,DepartmentofVeterinaryPreventiveMedicine,OhioAgriculturalResear
2、chandDevelopmentCenter,TheOhioStateUniversity,Wooster,OH44691,U.S.A.(Received27August1997/Accepted19January1998)ABSTRACT.Thediversityinselectedregionsofthetransmissiblegastroenteritisvirus(TGEV)andporcinerespiratorycoronavirus(PRCV)genomeswasanalyzedamongknownTGEVandPRCVstrainsandfieldisolates.TheN-
3、terminalhalfofthespike(S)glycoproteingeneandopenreadingframes(ORF)3,3–1and4wereamplifiedbyreversetranscriptasereactionandpolymerasechainreaction(RT/PCR),andanalyzedusingrestrictionfragmentlengthpolymorphism(RFLP)patternsoftheamplifiedDNA.ReferenceTGEVstrains(MillerandPurdue)andaPRCVstrain(ISU-1),and
4、TGEVandPRCVfieldisolateswereanalyzed.BasedonthesizeoftheORF3,3–1and4RT/PCRproducts,TGEVandPRCVstrainscouldbequicklyandeasilydifferentiatedintothreegroupsdesignatedTGEVMiller,PurduetypesandPRCV.ByRFLPanalysisoftheN-terminalregionoftheSglycoproteingeneandORFs3,3–1and4,TGEVandPRCVstrainsweredifferentia
5、tedintofivegroupsusingtherestrictionenzymeSau3AI.SequenceanalysisofaPCRproductintheORFs3,3–1and4fromvirulentandattenuatedMillerstrainsdemonstratedadditionaldifferencesinthatregionwhichhavebeencorrelatedwithachangeinvirulenceofTGEVisolates.—KEYWORDS:coronavirus,PRCV,RT/PCR-RFLPanalysis,TGEV.J.Vet.Med
6、.Sci.60(5):589–597,1998Transmissiblegastroenteritisvirus(TGEV)istheproposedasaviralmembrane-associatedminorstructuraletiologicalagentoftransmissiblegastroenteritis(TEG),apolypeptide[13].OthermRNAs,3,5,and8areassumedtohighlycontagiousentericviraldiseaseofswine[34].TGEVencodenonstructuralproteins.belo
7、ngstothefamilyCoronaviridae.ItisapleomorphicPorcinerespiratorycoronavirus(PRCV)hasbeenisolatedenvelopedviruswithalarge,single-stranded,positiveRNAfrompigsinEuropeandmorerecentlyintheUnitedStatesgenome
