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1、JournalofGeneralVirology(1996),77,2515-2521.PrintedinGreatBritainCharacterizationoffunctionaldomainsinthehumancoronavirusHCV229EreceptorAndreasF.Kolb,JuliaMaile,AngelienHeisterandStuartG.SiddellInstituteofVirologyandImmunology,UniversityofWOrzburg,Versb
2、acherstr.7,97078Worzburg,GermanyHumanaminopeptidaseN(hAPNorCD13)andsmallregionofpAPNsequence(pAPNaminoacidsporcineaminopeptidaseN(pAPN)arefunctional255-348)withthecorrespondinghAPNsequencereceptorsforhumancoronavirus(HCV)229Eand(hAPNaminoacids260-353)co
3、nvertspAPNintoaporcinetransmissiblegastroenteritisvirus(TGEV),functionalreceptorforHCV229E.Theregionofrespectively.However,hAPNcannotfunctionasahAPNthatwehavedefinedinthiswaydoesnotreceptorforTGEVandpAPNcannotfunctionasacorrespondtotheregionofpAPNthatha
4、sbeenreceptorforHCV229E.Inthisstudy,weconstructedidentifiedasessentialfortheTGEV-receptorin-aseriesofchimerichAPN/pAPNgenesandteraction.Weconcludethatalthoughbothvirusesexpressedthecorrespondingproteinsintrans-useahomologousreceptorprotein,differentfect
5、edcells.Subsequently,weidentifiedtheregionsoftheproteinarerequiredtomediatechimericproteinsthatcanfunctionasareceptorforsusceptibilitytoinfectionwithHCV229EandTGEV.HCV229E.TheresultsshowthatreplacementofaIntroductioninthemetabolismofregulatorypeptides(L
6、ooketal.,1989).Thehumancoronavirus(HCV)229EandtheporcineTheinteractionofaviruswithitscellularreceptorisoneofthecoronavirustransmissiblegastroenteritisvirus(TGEV)aremajortargetsofthehostimmuneresponseduringaviruscloselyrelatedandareantigenicallyandgeneti
7、callydistinctinfection.Neutralizingantibodieswhichinterferewithvirus-fromtheso-called'haemagglutinating'coronaviruses,suchasreceptorinteractionareabletoblocktheinfectionprocessinmurinehepatitisvirus(MHV)andbovinecoronavirus(BCV),vitroandinvivo(Dimmock,1
8、993).However,thevirus-andtheaviancoronavirusinfectiousbronchitisvirus(IBV)receptorinteractionisalsoapossibletargetforthede-(Siddell,1995).Therefore,notsurprisingly,infectionofcellsvelopmentofsyntheticanti-viraldrugs.Thisisexempli
