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1、J.gen.Virol.(1982),60,307-314.PrintedinGreatBritain307Keywords:coronavirus/macrophages/electronmicroscopyReplicationofHumanRespiratoryCoronavirusStrain229EinHumanMacrophagesByS.PATTERSON*ANDM.R.MACNAUGHTONElectronMicroscopyResearchGroup,SectionofHistopathologyandDivisionofCommunicableDis
2、eases,ClinicalResearchCentre,WatfordRoad,Harrow,MiddlesexHA13UJ,U.K.(Accepted21January1982)SUMMARYEvidenceforthereplicationofhumancoronavirusstrain229E(HCV229E)inmacrophagesispresented.Virusantigenwasdetectedinmacrophagesbyanimmunofluorescenttechnique24hafterinfectionandvirusparticleswer
3、eobservedinthecisternaeoftheendoplasmicreticulumbyelectronmicroscopy.Giantcellswereobservedbylightandscanningelectronmicroscopy,andlargemultinucleatecellswereseenbythin-sectionelectronmicroscopy,suggestingthatHCV229Ecaninducesyncytialformationinculturedhumanmacrophages.Furthermore,thepro
4、-ductionofinfectiousvirusbymacrophageswasdemonstratedbyaninfectiouscentreassay.INTRODUCTIONThecellsofthereticuloendothelialsystemplayakeyroleindeterminingsusceptibilitytovirusinfections(Mires,1964;Silverstein,1970;Morgensen,1979).Studieswithanumberofdifferentviruseshaveshownacorrelationb
5、etweenthegrowthofvirusinmacrophagesandthesusceptibilityofthehosttoinfection(Bang&Warwick,1960;Roberts,1963,1964;Tosolini&Mires,1971;Virelizier&Allison,1976).Coronavirusinfectionsoftherespiratorytractarewidespreadinthehumanpopulationandaccountforasignificantproportionofcolds(McIntosh,1974
6、;Monto,1974).However,therearefewdataavailableconcerningtheinteractionsbetweenhumanrespiratorycoronavirusesandmacrophages.Inviewoftheimportanceofmacrophagesinvirusimmunity,theinteractionsbetweenthehumanrespira-torycoronavirusesstrain229E(HCV229E)andhumanmacrophageshavebeeninvestigated.The
7、resultsofimmunofluorescence,electronmicroscopicandinfectivitystudiesonhumanmacrophagesinfectedinvitroshowthatthesecellscansupportthereplicationofHCV229E.METHODSVirus.HCV229EwasgrowninmonolayerculturesofdiploidcellsoftheMRCcontinuous(MRCc)lineasdescribedpreviously(Macnaugh
