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1、ArchVirol(2001)146:1219–1230GenomicandantigenicvariationsoftheHEglycoproteinofbovinecoronavirusesassociatedwithneonatalcalfdiarrheaandwinterdysenteryBriefReportA.B.Kourtesis,A.-M.Gélinas,andS.DeaCentreofMicrobiologyandBiotechnology,INRS-InstitutArmandFrappier,UniversitéduQ
2、uébec,Laval,Québec,CanadaAcceptedJanuary9,2001Summary.Inthisstudy,weattemptedtode?nedifferencesinthehemagglutinin-esterase(HE)glycoproteinbetween11bovinecoronaviruses(BCV)recent(post-1991)andpast(pre-1991)isolatesfromneonatalcalfdiarrhoea(NCD)andwinterdysentery(WD)syndrome
3、sasabasisforstraindifferentiationrelatedtotheclin-icalsourceoftheisolates.The?veWD-associatedBCVisolatesstudiedcouldbedistinguishedfrompastNCD-isolatesbytheirhemagglutinating(HA)prop-ertiesat4and37C,theirreceptor-destroyingenzyme(RDE)activitieswithraterythrocytesandlacko
4、freactivityoftheseNCDisolatestofourHAinhibiting(HAI)monoclonalantibodies(MAbs)directedagainsttheHEglycoproteinofthereferenceWD-associatedBCQ.2590Quebecstrain.Althoughminorornodif-ferencescouldbedemonstratedbycomparingbiologicalpropertiesoftheHEofWD-isolatestothoseofrecentN
5、CD-isolates,pastNCDisolateslackedreactivitywiththeWDHAIMAbs,whereasrecentNCDisolatesdisplayedtwodistinctre-activitypatterns.Attemptstode?nesequencedifferencesintheHEgenesoftheWDandNCDstrainsrevealedhighnucleotide(NT)identitieswithonlyscatteredaminoaciddifferences,seemingly
6、unrelatedtotheclinicaloriginoftheisolatesorHAIMAbreactivities.*Bovinecoronavirus(BCV)isanimportantenteropathogenicagentofthebovinespecieswhichreplicatesinthematureenterocytesofthesmallintestineandcolon,causingseverediarrhoeainneonatalcalves(NCD)[11,16,27]andchronicshedding
7、inadultcattle(AD)[4,12].Thevirushasbeenreportedtobealsoresponsibleforacuteentericinfectioninadultcattleduringthewinterseason1220A.B.Kourtesisetal.[1,8,21].Thedisease,usuallyreferredtoaswinterdysentery(WD),ischarac-terizedbyanacuteonsetofdark,liquid,hemorrhagicdiarrhoeaacco
8、mpaniedbydecreasedmilkproductionandvariabledepressionandanorexiainadultcattle.Other?eldis
