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1、稀有放線菌的分離與培養(yǎng)報(bào)告人：張曉敏報(bào)告內(nèi)容分離培養(yǎng)基的選擇小單孢菌形態(tài)與生理生化特征擬諾卡氏菌形態(tài)與生理生化特征小單孢菌、擬諾卡氏菌與鏈霉菌區(qū)別分離培養(yǎng)基的選擇淀粉-酪蛋白硝酸鹽瓊脂培養(yǎng)基溶膠幾丁質(zhì)瓊脂培養(yǎng)基甘露醇-利福平瓊脂培養(yǎng)基淀粉-酪蛋白硝酸鹽瓊脂培養(yǎng)基starch-caseinnitrateagar：starch10g，sodiumcaseinate0.3g，KNO32g，agar15g，PH7.0-7.4.37μgnovobiocin，223μgmycostatin30℃培養(yǎng)21天Micromonosporachaiyaphumensissp.novMi
2、cromonosporaeburneasp.nov基礎(chǔ)培養(yǎng)基：K2HPO40.7g，KH2PO40.3g，MgSO4·7H2O0.5g，F(xiàn)eSO4·7H2O0.01g，ZnSO40.001g，瓊脂10克，蒸餾水1.0L.PH7.0121℃蒸汽滅菌20min,每100mL加20mL膠體幾丁質(zhì)懸浮液和5mg放線菌酮溶膠幾丁質(zhì)瓊脂培養(yǎng)基甘露醇-利福平瓊脂培養(yǎng)基mannitol-rifampicinagar：甘露醇10g，酵母提取物7g，酪蛋白水解物2g，蛋白胨1g，NaCl1g，CaCO30.2g，制霉菌素100mg,利福平5mg27℃培養(yǎng)6周Nocardiaacidiv
3、oran返回MicromonosporagyrB基因序列分析和DNA–DNA雜交15species:Micromonosporaaurantiaca,Micromonosporacarbonacea,Micromonosporachalcea,M.chersina,Micromonosporacoerulea,Micromonosporaechinospora,Micromonosporagallica,Micromonosporahalophytica,Micromonosporainositola,M.matsumotoense,Micromonosporani
4、gra,M.olivasterospora,Micromonosporapallida,MicromonosporapurpureochromogenesandM.rosaria.小單孢菌形態(tài)與生理生化特征Aerobic,Gram-positive,mesophilic,non-motileactinomycetewell-developedandbranchedsubstratehyphae.Aerialmyceliumwasabsent.Sporechainswereabsent.Mycolicacidswereabsent.Acyltypeofcellwall
5、muramicacidisglycolyl.Fatty-acidtype:3b.Thepredominantmenaquinone:MK-9（H4）、MK-9（H4H6H8）Characteristicwhole-cellsugarsarexyloseandarabinose.Phospholipids:P-II型.Cell-wallpeptidoglycan:CW-II(glutamicacid,alanineandmeso-diaminopimelicacid).DNAG+C(mol%):71%-73%.>well-developedandbranchedsub
6、stratehyphae.>Aerialmyceliumwasabsent.>Atmaturity,sporesweresphericaltooval,appearedtoberoughandwerenon-motile(Fig.1).>colourofthevegetativemyceliumonISP2mediumisorange,turningtogreyishblackorblackuponsporulation.>growthatpH4-8and25-30℃,nogrowthabove45℃>Brown,diffusiblepigment>positive
7、fortheutilizationofL-arabinose,D-fructose,D-galactose,D-glucose,lactose,melibiose,salicinandD-xylose.>negativefornitratereductionandtheutilizationofD-mannitolandrhamnose.Micromonosporachaiyaphumensis>Single,non-motile,sphericalspores(0.6–0.9mm)wereformedonsubstratehyphae.>Aerialmycel
