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1、：營養(yǎng)1鬟健s面andcFoodIndus#y納豆激酶對(duì)角叉菜膠所致小鼠血栓模型的影響劉彥霞，陳文，溫宗妍，趙肖萌(北京聯(lián)合大學(xué)應(yīng)用文理學(xué)院，生物活性物質(zhì)與功能食品北京市重點(diǎn)實(shí)驗(yàn)室，北京100191)摘要：目的：觀察納豆激酶對(duì)角又菜膠所致的小鼠血栓模型的影響，為納豆激酶相關(guān)產(chǎn)品的溶血栓功能性評(píng)價(jià)提供依據(jù)。方法：將雌性昆明種小鼠隨機(jī)分為4組：空白組、模型對(duì)照組、低劑量組、高劑量組。連續(xù)灌胃30d，每天一次．于第30d，除空白組外，其他3組均用角又萊膠制備尾部血栓模型，24h后觀察并測(cè)量黑尾長度．之后摘眼球取血，測(cè)定血清纖維蛋白降解產(chǎn)物(FDP)、組織型纖溶酶原激活劑(t—P
2、A)、組織型纖溶酶原激活劑抑制劑l(PAI一1)的含量。結(jié)果：各組小鼠最后一次稱重體重?zé)o顯著性差異；低劑量組與高劑量組的出栓率低于模型對(duì)照組，但模型對(duì)照組、低劑量組、高劑量組三組間相比較，黑尾長度、黑尾／全尾長的無顯著性差異(p>0．05)；高劑量組小鼠血清中的FDP~t—PA的舍量顯著高于模型對(duì)照組和低劑量組(p<0．05)；空白組未檢出PAI一1，其余各組PAI一1的含量無顯著性差異。結(jié)論：納豆激酶對(duì)實(shí)驗(yàn)小鼠體內(nèi)血栓具有溶解作用，可以降低血栓的發(fā)生率；角叉萊膠誘導(dǎo)的小鼠血栓模型可以用于納豆激酶溶(抗)血栓功能評(píng)價(jià)。關(guān)鍵詞：納豆激酶，角叉萊膠．小鼠．尾部血栓Impact
3、ofnattokinaseoncarrageenaninducedtail-thrombusmiceLIUYan-xia，CHENWen，WENZong-yan，ZHAOXiao—meng(BeijingKeyLaboratoryofBioaetiveSubstancesandFunctionalFoods，CollegeofArtsandScience，BeijingUnionUniversity，Beijing100191，China)Abstract：Objective：Observedefectofnattokinaseonthetail—thrombusmode
4、I，andprovidedbasisforthefunctionevaluationofnattokinaseproduct．Methods：Themicewererandomlyallocatedjntofourgroups(eachgroupwith12mice)，includingthenormalgroup，themodelcontrolgroup，thelow—dosenattokinasegroup，andthehigh-dosenattokinasegroup．Weighingupthemicewasdoneevery7d．Afterthemiceweref
5、edfor30d，exceptforthenormalgroup，other3groupswereusedtoestablishtaft-thrombusmodelswithcarrageenanAftermodelswereestablishedfor24h．weobservedthelengthOfdarktails．a(chǎn)ndthendetectedtheFibrinogendegradationproduct(FDP)，tissuetypeplasminogenactivator(t-PA)，andtissuetypeplasminogenactivatorinhib
6、itor(PAl一1)inserumResults：Therewasnosignificantdifferencebetweenthelengthofdarktailsamongthemodelcontrolgroup．thelow-dosenattokinasegroupandthehigh-dosenattokinasegroup(p>0．05)，butthedarktailratesofthelatertwogroupwereIowerthanthefOrmerone．ThevaluesofFDPandt—PAofthehiqh—dosenattokinasegro
7、upwerehigherthanthoseofthemodeicontrolgroupandtheIOW—dosenattokinasegroup(p<0．05)．noPAl-1wasdetectedinthenormal，andothergroupshadsimilarvaluesofPAl一1．Conclusions=Nattokinasewasefectiveindissolvingbloodclots，anddroppingincidenceofdisease．Carrageenaninducedtail—thromb
