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《玉米-大豆輪作及氮肥施用對(duì)土壤細(xì)菌群落結(jié)構(gòu)的影響-論文.pdf》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫(kù)。
1、作物學(xué)報(bào)ACTAAGRONOMICAS~ICA2013，39(11)：2016-2022http：／／zwxb．chinacrops．org／ISSN0496—3490；CODENTSHPA9E—mail：xbzw@chinajourna1．net．caDoI：10．3724／SP．J．1006．2013．02016玉米．大豆輪作及氮肥施用對(duì)土壤細(xì)菌群落結(jié)構(gòu)的影響周嵐，2，楊永，王占海陳阜曾昭海，中國(guó)農(nóng)業(yè)大學(xué)農(nóng)學(xué)與生物技術(shù)學(xué)院，北京100193；吉林農(nóng)業(yè)科技學(xué)院，吉林吉林132101；呼倫貝爾市農(nóng)業(yè)科學(xué)研究所，內(nèi)蒙古扎蘭屯162650摘要：在大豆開(kāi)花期分
2、別對(duì)3個(gè)施氮水平下(0、50和100kghm-2)大豆連作(大豆一大豆．大豆)、玉米．大豆輪作I(大豆．玉米．大豆)及玉米一大豆輪作II(玉米．玉米．大豆)，應(yīng)用PCRDGGE技術(shù)研究玉米．大豆輪作及施氮對(duì)土壤細(xì)菌群落結(jié)構(gòu)變化的影響。結(jié)果表明，隨著施氮水平的提高，3種種植方式土壤中細(xì)菌群落多樣性、豐富度均呈減少趨勢(shì)。高氮處理(100kghm-2)明顯降低了大豆連作、玉米一大豆輪作II根際土壤細(xì)菌群落多樣性及豐富度，玉米一大豆輪作I根際土壤細(xì)菌群落多樣性及豐富度略有降低。玉米一大豆輪作1種植方式可減輕氮肥對(duì)其根際細(xì)菌群落多樣性和豐富度的影響，但施氮明顯改變
3、了其細(xì)菌群落結(jié)構(gòu)。玉米一大豆輪作II中大豆根際土壤細(xì)菌群落結(jié)構(gòu)較為穩(wěn)定，受氮肥影響較小。在3種種植方式的土壤中，分布著酸桿菌門(mén)、變形菌門(mén)及厚壁菌門(mén)細(xì)菌，其中前兩門(mén)菌群占主導(dǎo)地位。關(guān)鍵詞：輪作；氮肥；大豆；玉米；DGGE；細(xì)菌群落InfluenceofMaize-SoybeanRotationandNFertilizeronBacterialCommunityCompositionZHOULan，，，YANGYong，，WANGZhan．Hai，CHENFu，andZENGZhao—Hai'CollegeofAgricultureandBioteclmol
4、ogy，Beijing100193，China；。JilinAgriculturalScienceandTechnologyCollege，Jilin132101，China；HulunbeierAgriculturalInstitute，Zhalantun162650，ChinaAbstract：Insoybeanfloweringperiod，tookrhizospheresoilsamplesfromthreetreatmentsincludingcontinuouscroppingsoy—bean(soybean—soybean—soybean)
5、，maize—soybeanrotationI(soybean—maize—soybean)andmaize—soybeanrotationII(maize—maize—soybean)．Eachtreatmenthadthreelevelsofnitrogenapplicationincluding0，50，and100kgha～．SoilmicrobialcOInlTIU—nitychangesundermaize-soybeanrotationsystemandvariousnitrogenapplicationswereinvestigatedb
6、ythetechniquesofdena-turinggradientgelelectrophoresis(DGGE)analysis．TheresultsshowedthatthebiologicaldiversityandabundanceofthreekindsofsoilmicrobialcommunityhadadeclineⅡ_endalongwiththeincreaseofnitrogenapplicationlevels．Underhighnitrogenappli—cationlevel(100kgha一)，asignificantd
7、ecreaseindiversityandabundanceofrhizospheresoilmicrobialcommunitieswasob．servedinbothcontinuouscroppingsoybeansystemandmaize—soybeanrotationIIsystem．Andinmaize—soybeanrotationIsystem，therewasonlyaslightlydecreaseindiversityandabundanceofrhizospheresoilmicrobialcommunities．Therefo
8、re，1-yearmaizeinrotationwithsoybean(soyb
