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1、·762·余宗艷，等地塞米松聯(lián)合順鉑對肺腺癌A549細胞增殖和凋亡的影響地塞米松聯(lián)合順鉑對肺腺癌A549細胞增殖和凋亡的影響余宗艷，齊宇紅，梁軍Efectsofdexamethasonecombinedwithcisplatinofproliferationandapoptosisofhu-manpulmonaryadenOCarCin0macellK549YuZongyan，QiYuhong，LiangJunDepartmentofRadiotherapy，TangduHospitalofthe4thMilitaryMedicalUniversity，ShaanxiXi'an7
2、10038，China．【Abstract】Objective：Toinvestigatetheeffectsofproliferationandapoptosisandthemechanisminducedbydexa-methasone(DEX)andcisplatin(DDP)inhumanpulmonaryadenoearcinomaceUA549andpossiblemechanism．Methods：SubculturehumanpulmonaryadenocarcinomacellA549invitro，andwastreatedwithDEXandDDP．Thep
3、roliferationabilityoftheA549ceilsweredetectedbyMTrmethod．Thecellcycleandcellapoptosiswereanalyzedbyfloweytometry．TheexpressionlevelofvitaminDreceptorwasevaluatedbyWesternblot．Results：Theconcentra-tionofDEXabove500nmo~LcouldinhibittheproliferationofA549cellsobviously，theinhibitoryratedependson
4、theconcentrationandtime(P<0．05)．TheconcentrationofDEXlessthan500nmol／Lcouldntinhibittheprolifera—tionofA549cells(P>0．05)．TheconcentrationofDDPin0．3125—10p,g／mlcouldinhibittheproliferationofA549cellsobviously，theinhibitoryratedependsOiltheconcentrationandtime(P<0．05)．Thecellcycleandcellapopto—
5、sisanalyzedbyflowcytometry，theDDP+DEXgroupG1phaseandSphasecellpercentagedropstorisemoreobvious(P<0．05)．WesternblotobservedtheVDRexpressionlevelofDDP+DEXgroupincreasedsignificantly(P<0．05)．Conclusion：DEXcombinedwithDDPcouldincreacetheexpressionofVDRontheproliferationofA549cells，andpromottheapo
6、ptosisandinducecellcyclearrest，andimprovethecisplatinchemotherapysensitivity．【Keywords】dexamethasone．humanpulmonaryadenocarcinomacellA549；cisplatin；cellcycle；chemotherapysensi—tivityModemOncology2014，22(04)：0762—0766【摘要】目的：探討地塞米松(dexamethasone，DEX)聯(lián)合順鉑(cisplatin，DDP)在體外對肺腺癌A549細胞增殖和凋亡的影響及可能的機
7、制。方法：體外培養(yǎng)人肺腺癌A549細胞。MTF法檢測DDP和DEX對A549細胞增殖的影響。流式細胞術(shù)分析細胞周期及細胞凋亡。Westernblot檢測DEX和DDP對A549細胞內(nèi)VDR表達水平的影響。結(jié)果：地塞米松>~500nmol／L濃度時對A549細胞有一定的增殖抑制作用(P<0．05)；<500nmoVL濃度時對A549細胞無明顯抑制作用(P>0．05)；DDP濃度在0．3125一lOng／ml范圍內(nèi)對A549細胞增殖有明顯的抑制作用，并呈濃度及時間依賴性(P<0．05
