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《β-欖香烯聯(lián)合順鉑對(duì)乳腺癌 MCF -7細(xì)胞增殖和凋亡的影響-論文.pdf》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫。
1、匡堂生月第22卷第5期MODERNONCOLOGY．Mav．2014．VOL．22．NO．05·1005·l3一欖香烯聯(lián)合順鉑對(duì)乳腺癌MCF一7細(xì)胞增殖和凋亡的影響鄭瑾，馬力天，任秦有，楊建棟，劉毅，張毅，范玉貞，張偉，張哲偉，瞿湘梅，楊明會(huì)Efectofp—ElemenecombinedwithDDPoftheproliferationandapoptosisofMCF——7celIZhengJin，MaLitian，RenQinyu，YangJiandong，LiuYi，ZhangYi，F(xiàn)anYuzhen，Zhang
2、Wei，ZhangZhewei，QuXiangmei，YangMinghuiDepartmentofTraditionalChineseMedicine，ChinesePGeneralHospital，Beijing100853，China；DepartmentIntegratedTraditionalandWesternMedicineofOncology，TangduHospital，eFourthMilitaryMedicalUniversity，ShaanxiXihn710038，China；StudentTea
3、ms，TheFourthMilitaryMedicalUniversity，Shaanxian710032，China；465547MilitaryHospital，LiaoningHaicheng114200，China．【Abstract】Objective：ToinvestigatetheefectofB—ElemenecombinedwithDDPontheproliferationandapop．tosisofMCF一7cel1．Methods：DifferentconcentrationsofB—Elemen
4、einjection(25，50，100，150，2001zg／m1)wereappliedtoMCF一7cells．CellproliferationwasmeasuredbyMTr．Cellapoptosisandcell—cyclewasdetectedbyflowcytometry．Theappropriatedrugconcentration(3tLg／mlDDP，125tLg／mlB—Elemene)wereselected．Then8一E1-emenecombinedwithDDPinectionwasap
5、pliedtoMTrcells．CellproliferationwasmeasuredbyMTF．Cellapoptosisandcell—cyclewasdetectedbyfloweytometry．Results：CellproliferationwasdramaticallyinhibitedbyB—Elemeneinjection(25，50，100，150，2001xg／m1)at24h，48h(P<0．05)．Inaddition，cellproliferationwasdramaticallyinhib
6、—itedbyinjectionB—ElemenecombinedwithDDP．Combinationtherapy，apoptosisrateandinhibitionrateofprolifera—tioninMCF一7cellstreatedbyp—E1emenecombinedwithDDPwashigherthanthemonotherapy(P<0．01)．Cell—cyclewasarrestedinGo／G1phasebyelemenecomparedtouninfeetiouscellsinthesa
7、metimescale．Conclusion：p—ElemeneandDDPcombinedwithp—Elemenecaninhibittheproliferation，acceleratetheapoptosisinMCF一7cells．DDPcombinedwithB—ElemenehavesynergisticeffectontheapoptosisinMCF一7cells．【Keywords】B—Elemene；DDP；MCF一7cell；proliferation；apoptosis；cell—cycleMo
8、dernOncology2014．22(05)：1005—1009【摘要】目的：探討B(tài)一欖香烯聯(lián)合順鉑對(duì)乳腺癌MCF一7細(xì)胞生長的影響。方法：體外培養(yǎng)乳腺癌MCF一7細(xì)胞，將B一欖香烯(濃度梯度為25、50、100、150、2001xg／m1)，單獨(dú)作用于乳腺癌MCF一7細(xì)胞，加藥24h、48h后用噻唑藍(lán)(MYr法)
