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1、第3l卷第3期生物醫(yī)學工程學雜志V0I．3lNo．32014年6月JournalofBiomedicalEngineeringJune2014地塞米松對順鉑誘導人肺腺癌細胞SPC—A1凋亡的抑制作用及分子機制曹菲張智慧1(成都市西區(qū)醫(yī)院腫瘤科，成都610036)2(四川省腫瘤醫(yī)院腫瘤內科，成都610041)摘要：研究地塞米松(DEX)對順鉑(cDDP)引起的人肺腺癌細胞SPC—A1凋亡的抑制作用及可能的分子機制。將不同濃度的DEX分別加入人肺腺癌細胞SPC—Al體外誘導培養(yǎng)24h后，再用不同濃度CDDP處理
2、SPC—A1細胞48h。MTT法檢測細胞存活率；RT—PCR方法檢測1tmol／IDEX誘導培養(yǎng)不同時間后SPC—A1細胞內血清／糖皮質激素一誘導激酶(SGK一1)和有絲分裂原蛋白激酶(MKP一1)的表達；用生物素標記的抗糖皮質激素受體(GR)抗體對SPC—A1細胞行免疫組化染色來檢測GR的表達。MTT測定結果顯示，SPC—A1細胞經DEX誘導后，對CDDP所致的凋亡有抵抗作用并與DEX濃度呈劑量依賴關系。RT—PCR檢測到DEX誘導培養(yǎng)能提高SGK—l在SPC—A1細胞中的表達，表達量隨時間延長而增加；但
3、未檢測到MKP一1的表達。免疫組化檢測顯示SPC—A1細胞經DEX誘導后GR上調，細胞內GR表達的陽性細胞數明顯高于對照組。結果表明DEX對CDDP引起SPC—A1細胞的凋亡有抑制作用?？赡艿姆肿訖C制是通過上調細胞內GR表達，進而上調其通路下游抗凋亡蛋白SGK一1的表達，導致SPC—Al細胞產生抗凋亡作用。關鍵詞：地塞米松；糖皮質激素受體；肺癌；化療；抗凋亡中圖分類號R73—36文獻標志碼ADOI：10．7507／1OO卜55l5．20140122TheInhibitoryEffectsofDexameth
4、asoneonCisplatinInducedApoptosisofHumanLungAdenocarcinomaCellSPC—A1andItsMolecularMechanismCAoFeiZHANGZhihui1(Departmento_，Ontology，ChengduWesternHospital，Chengdu610036，China)2(Department0，MedicalOmology，SichuanProvincialTumorHospital，Chengdu610041，China)A
5、bstract：Theaimofthisstudyistoinvestigatetheapoptoticinhibitionanditsmolecularmechanismofdexametha—sone(DEX)actingoncisplatin(CDDP)一inducedapoptosisofhumanlungadenocarcinomacellSPC—A1．SPC—A1ceilswerepre—culturedinvitrofor24hourswithDEXindifferentconcentrati
6、onsandthenCDDPwasaddedindifferentconcentrationsforculturingforfurther48hours。ThesurvivalratesofthecellsweredeterminedbyMTT．Theex—pressionofserum／glucocorticoid—inducedkinase(SGK一1)andmitogen—activatedproteinkinasephosphatase一1(MKP一1)inSPC—A1ceilsa{terbeing
7、culturedby1~mol／IDEXatdifferenttimewasdetectedbysemi—quantitativeRT～PCRtechnology．Theexpressionofglucocorticoidreceptor(GR)inSPC—A1cellswasmeasuredbyimmunohisto—chemistry(IHC)withbiotin—labeledanti—GR．TheresultsofMTTshowedthatSPC—AlcellshadresistancetoCDDP
8、inducedapoptosiswithpre—culturedDEXandtheresistanceintensitypresentedDEXconcentration-dependent．TheexpressingquantityofSGK一1inSPC—A1cellsstimulatedbyDEXcouldbeelevatedandincreasedwithintentionoftime．buttheexp
