資源描述：
《基因芯片對(duì)哮喘小鼠趨化因子及其受體差異表達(dá)的篩選與分析-論文.pdf》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在應(yīng)用文檔-天天文庫。
1、第23071卷4年6第月3期遵義醫(yī)學(xué)院學(xué)報(bào)VOl-37NO．3JournalofZunyiMedicalUniversityJun．2014基因芯片對(duì)哮喘小鼠趨化因子及其受體差異表達(dá)的篩選與分析岳歡，李燕，高婧，江濤，黃俊瓊(1．遵義醫(yī)學(xué)院附屬醫(yī)院檢驗(yàn)科／遵義醫(yī)學(xué)院檢驗(yàn)系，貴州遵義563099；Z四川省仁壽縣疾病預(yù)防控制中心，I~Jll眉山6~5oo)[摘要]目的用SABioscience基因芯片檢測(cè)小鼠哮喘模型中84個(gè)趨化因子及其受體基因，并初步篩選分析其與支氣管哮喘的相關(guān)性。方法用OVA構(gòu)建哮喘小鼠模型，于末次激發(fā)后取右肺組織進(jìn)行HE染色，并提取左肺組織總RN
2、A，用SABioscience芯片檢測(cè)84個(gè)趨化因子及其受體基因的表達(dá)水平。結(jié)果成功構(gòu)建哮喘小鼠模型，哮喘組與對(duì)照組相比，表達(dá)上調(diào)的趨化因子21個(gè)，趨化因子受體l3個(gè)；表達(dá)下調(diào)的趨化因子4個(gè)。結(jié)論SABioscience芯片可有效地篩選出哮喘小鼠差異表達(dá)的趨化因子及其受體，對(duì)進(jìn)一步深入探討哮喘的發(fā)病機(jī)制及預(yù)防具有重要意義。[關(guān)鍵詞]支氣管哮喘；趨化因子；受體；基因芯片[中圖法分類號(hào)]R392．8[文獻(xiàn)標(biāo)志碼]A[文章編號(hào)]1000-2715(2014)03-0286-04Screeningandanalysisofchemokinesandtheirrecepto
3、rsexpressioninmurineasthmamodelusinggenemicroarrayYueHuan，LiYah，Gao，JiangTao，HuangJunqiong(1．DepartmentofClinicalLaboratory．ClinicalLaboratory，theAmliatedHospitalofZunyiMedicalUniversity／DepartmentofLaboratoryMedicine，ZunyiMedicalUniversity，ZunyiGuizhou563099，China；2．RenshouCenterforD
4、iseaseControlandPrevention，MeishanSichuan620500，China)[Abstract]ObjectiveTotesttheexpressionof84chemokinesandchemokinesreceptorsgenesinmurineasth-mamodelusingSABiosciencegenemieroarrayandpreliminarilyscreenandanalyzetheirrelationshiptoasth—ma．MethodsMurineasthmamodelwasestablishedthro
5、ughi．P．injectionofOVAandaerosolchallenge．Within24hoursafter—laststimulating，therightlungtissueofmicewasstainedbyHEstaining．TotalRNAwasextrac—tedfromleftlungtissueandtheexpressionlevelof84chemokinesandchemokinereceptorswereanalyzedbySABiosciencegenemicroarray．ResultsMurineasthmamodelsw
6、assuccessfullyestablished．21chemokinesand16chemokinereceptorsareovertwofoldup—regulatedand4chemokinesareovertwofolddown—regulatedinasthmagroup，comparedwiththoseincontrolgroup．ConclusionDifferentlyexpressedchemokinesandchemo—kinereceptorsofasthmamicecouldbeeffectivelyfilteredoutbySABio
7、sciencemicroarray．whichprovidedthefoundationforfurtherexplorationofthepathogenesismechanismandpreventionofasthma．[Keywords]asthma；chemokine；receptor；genemicroarray趨化因子是一類小分子可溶性蛋白，能使免疫模型的表達(dá)情況，為進(jìn)一步研究其功能奠定基礎(chǔ)。細(xì)胞發(fā)生定向趨化和遷移，在炎癥反應(yīng)和免疫應(yīng)答中具有重要作用。哮喘疾病中炎性細(xì)胞的激活和1材料與方法浸潤，釋放趨化因子，調(diào)控其受體的表達(dá)，從而誘導(dǎo)1．1主要試劑雞
8、卵白蛋白(
