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1、nCT^nBRICULTURnE華北農(nóng)學(xué)報(bào)·2008，23(增刊)：i29．132B0REnLl—SIHICn分子標(biāo)記技術(shù)在咖啡育種上的應(yīng)用李國鵬，何紅艷，黃家雄，羅心平，李貴平，楊仕貴，李樹維，黃健，郭容琦(云南省農(nóng)科院熱帶亞熱帶經(jīng)濟(jì)作物研究所，云南保山678025)摘要：主要介紹了AFLP和RAPD2種分子標(biāo)記技術(shù)以及2種分子標(biāo)記的優(yōu)缺點(diǎn)。同時(shí)，著重介紹了2種分子標(biāo)記在咖啡育種上的應(yīng)用成果。在最后還列舉了2個(gè)其他的分子標(biāo)記在咖啡育種上的應(yīng)用。關(guān)鍵詞：咖啡；分子標(biāo)記技術(shù)；AFLP；RAPD；應(yīng)用中圖分類號(hào)：Q75文獻(xiàn)標(biāo)識(shí)碼：A文章編號(hào)：1000—7091(2008)~刊一0129—
2、04MoleculeMarkerTechnologyinCoffeeBreedingApplicationLIGuo—peng，HEHong—yan，HUANGJia—xiong，LUOXin—ping，LIGui—ping，YANGShi—gui，LIShu—wei，HUANGJian，GUORong—qi(TropicalandSubtropicalCashCropsInstituteofYunnanAgricultureScienceResearchInstitute，Baoshan678025，China)Abstract：ThisarticleintroducedAFLPa
3、ndRAPDtechnologies，anditsapplicationincofeebreedingprogram．ThisarticleintroducedthemeritanddisadvantagesoftheAFLPandRAPD．Attheend，gave2samplesofothermoleculemarkertechnologyincoffeebreeding．Keywords：Coffee：Moleculemarkertechnology；AFLP；RAPD；ApplicationCofleeandteaarethetwomostimportantbeverages
4、Inrecentyears，DNAmolecularmarkerssuchasoftheworld．AlthoughthegenusCoffeecontainsabout80AFLP，RAPDandISSR，whichareindependentofenvi—taxa，commercialcofeeproductionisbasedontwospeciesronmentalinfluences，havebeenusedinthecoffeebreed．i．e．C．a(chǎn)rabica(2n=4x=44)andC．canephora(2n：ingresearch．Inthisarticle，
5、wejustintroducedtheprinci—2x=22)．Theyaccountingrespectivelyforabout70％andplesofsomemodernbio—technologiesanditsapplication30％oftheworldproduction．TheArabicacofeecultiva—onthecoffeebreedingresearch．tionstartedinArabia，specificallyinYemen，fivecenturies1Thebriefintroductionandutilizationofago．Inth
6、eearly18thcentury，progeniesfromasingleIn—AFLPincoffeebreedingdonesiaplantcultivatedinEuropewerespreadouttoAmplifiedFragmentLengthPolymorphisms(AFLP)SouthAmericaandturnedouttobethegeneticbasisofisbasedontheselectivePCRamplificationofrestrictionmaincuhivamofBrazilandothercountries．Severalcen·frag
7、mentsfromatotaldigestofgenomicDNA．AndAFLPturiesago，theaimofcropbreedingjustemphasisonhighisaDNAfingerprintanalysismethodwithhighdiscrimi—yield．Nowadays，thebreedingprogramarecenteringonnatorycapacity．Comparedtoothermarkertechniques
