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1、-BT●8勰骺華北農(nóng)學(xué)報·2010,25(2):236-238山藥多糖對PRRSV滅活苗免疫豬抗體和T細(xì)胞亞群的影響張紅英,王學(xué)兵,崔保安,趙現(xiàn)敏,陳紅英,金鉞(1.河南農(nóng)業(yè)大學(xué)牧醫(yī)工程學(xué)院,河南鄭州450002;2.河南省動物性食品安全重點實驗室,河南鄭州450002)摘要:研究山藥多糖對PRRSV滅活苗免疫抗體和豬外周血T細(xì)胞亞群的影響。選擇35日齡斷奶仔豬l6只,隨機(jī)分為4組,將山藥多糖分別以17.1mr,/kg和8.55ms/kg兩個劑量和豬PRRSV滅活苗同時注射,于免疫后7,14,24,34,44,54,69,79d采血,ELI
2、SA方法檢測豬PRRSV抗體水平,流式細(xì)胞術(shù)檢測豬外周血中T淋巴細(xì)胞亞群的變化。結(jié)果表明,山藥多糖可顯著提高PRRSV滅活苗免疫豬外周血CD3和CD8細(xì)胞數(shù)量,在免疫34d后,兩個劑量的山藥多糖均可顯著提高PRRSV滅活苗免疫抗體水平。山藥多糖可以作為免疫增強(qiáng)劑與PRRSV滅活苗聯(lián)合使用。關(guān)鍵詞:山藥多糖;PRRSV滅活苗;抗體;T淋巴細(xì)胞亞群中圖分類號:$852.65文獻(xiàn)標(biāo)識碼:A文章編號:1000—7091(2010)02—0236—03EffectsofChineseYamP0lvsaccharideonAntibodyandTLym
3、phocytesSubpOpulatiOnsintheImmuneResponsetoPRRSVKilledVaccineZHANGHong—ying’,WANGXue—bing,,CUIBao—an,,ZHAOXian.min,CHENHong—ying‘,JINYue(1.CollegeofAnimalHusbandryandVeterinaryMedicine,HenanAgriculturalUniversity,Zhengzhou450002,China;2.HenanKeyLaboratoryforAnima1.derivedF
4、oodSafety,Zhengzhou450002。China)Abstract:TheresearchistostudytheeffectsofChineseyampolysaccharide(CYPS)onantibodyandTsubpopu-lationsinpigletspheripheralbloodintheimmuneresponsetoPRRSVkilledvirusvaccine.1635·day-oldpigletswererandomlyassignedintoequalfourgroupsandthreegroup
5、swerevaccinatedwithPRRSVkilledvirusvaccine.Atthesametime,thepigletsingroupsCYPSHandCYPSLwereintramuscularlyinjectedwithCYPSat17.1or8.55mg/kgdosages,groupC(controlgroup),withsterilesaline,onceadayforthreesuccessivedays,groupF(fakegroup)onlyinjectedwithsaline.At7,14,24,34,44
6、,54,69,79dayaftervaccination,pheripheralbloodweresampledfordetectionofserumantibodytitertoPRRSVbyELISAandTlymphocytessubpopulationsbycytofluorometry.ResultsshowedthatCYPScanboosttheproportionofCD3andCD8lymphocytesinpigletspheripheralbloodafterimmunizationwithPRRSVkilledvir
7、usvaccine,andenhancedantibodytitertoPRRSVafter34days.CYPSisapotentialimmunostimula-toryforPRRSVkilledvirusvaccine.Keywords:CYPS;PRRSVkilledvirusvaccine;Antibody;Tlymphocytesubpopulations山藥為薯蕷科多年生宿根蔓草植物山藥(D—免疫力¨’,但山藥多糖對豬是否同樣具有免疫促oscoresoppositaThunb)的塊根,又名白苕、土薯、大進(jìn)作用尚未見報道。薯、
8、薯藥,具有補(bǔ)脾、益肺、固腎、益精的作用,原名薯豬繁殖與呼吸綜合征(Porcinereproductiveand蕷,首見于《神農(nóng)本草經(jīng)》。據(jù)《本草綱目》記載,山respirator