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1、南方農(nóng)業(yè)學(xué)報(bào)JOuRNAL0FsouTHERNAGRIcuIJuREISSN2095一l191:CODENNNXAABhttp:/,hww.mhyxb.comDOI:10.3969/j:issn.2095—1191.2叭2.02.249豬流感病毒H1N2亞型油乳劑滅活苗的免疫效果陸增榮雌,張民秀1,黃偉堅(jiān)”,侯韶毅3,盧桂娟z,肖雄1,黃福標(biāo)1,盧冰霞1,郭旋1,劉磊1,趙武。,陳澤祥4(1廣西大學(xué)動(dòng)物科學(xué)技術(shù)學(xué)院,南寧530005;2廣西麗原生物股份有限公司,南寧53000l;3玉林市動(dòng)物疫病
2、預(yù)防控制中心廣西玉林537000,4廣西獸醫(yī)研究所,南寧530001)摘要:【目的】評(píng)估以H1N2亞型豬流感病毒(sIv)制成油乳劑滅活苗的免疫效果,為預(yù)防sIV的發(fā)生和蔓延提供參考依據(jù)。【方法】采用不同劑量的sIvH1H2亞型油乳劑滅活苗對(duì)豬只進(jìn)行免疫,免疫后采血,用HI試驗(yàn)測(cè)定抗體效價(jià),連續(xù)測(cè)定至第10周,然后對(duì)豬只進(jìn)行攻毒,并觀察其臨床癥狀及進(jìn)行病毒檢測(cè)等?!窘Y(jié)果】中免疫劑量組(4mL/頭)和高免疫劑量組(6mI/頭)的抗體滴度高于低免疫劑量組(2mL/頭);免疫組與對(duì)照組在攻毒后豬只的體
3、溫?zé)o顯著差異(P>0.05),免疫組攻毒后無(wú)明顯的臨床癥狀,除中免疫劑量組在攻毒后第1d檢測(cè)出鼻粘液病毒外,其他時(shí)間及其他劑量組均未檢測(cè)到sIv,對(duì)豬只起到良好的保護(hù)作用?!窘Y(jié)論】sIVH1N2亞型油乳劑滅活苗具有良好的免疫效果,對(duì)同亞型病毒的攻擊具有一定保護(hù)作用,其最佳免疫劑量為4mL/頭。關(guān)鍵詞:豬流感病毒;H1N2亞型;油乳劑滅活苗;免疫;攻毒中圖分類號(hào):S858.28文獻(xiàn)標(biāo)志碼:A文章編號(hào):2095—119l(2012)02—0249—04Inlmunizationefflect0fin
4、actiVatedoil—emulsionVaccineonpigsinfectedwithH1N2subtypeswineinnuenzaVirusLUZeng—rong1”,ZHANGMin—xiul,HUANGWei-jian”,HOUShao—yi3,LUGui-juan2,XIAOXiong1,HUANGFu—bia01,LUBing—xial,GUOXuanl,LIULeil,ZHAOWu4,CHENZe—xian94(1CollegeofAnimalScienceandTechn0
5、109)r,Guang)【iUniversity,Nanning530005,China;2Guan黟i“yuanBiologyCo.,Ltd,Nanning530001,China;3YulinCenterforAnimalDiseasePreventionandControl,Yulin,Guangxi537000,China;4GuangxiVeterinaryResearchInstitute,Nanning530001,China)Abstract:【0biective】TheeⅡbc
6、tofinactivatedoil—emulsionvaccineforswineinnuenzavims(SIV)H1N2subtypewasevaluatedinordertopreventtheoccurrenceandspreadofsIVemciently.【Method】7IheimmunizedpigswereinjectedintramuscularlywithdiffeI℃ntdoses(2,4and6mLperpig)ofinactivatedH1N2vims,andtheb
7、loodofpigswerecoUect—edandthetiterofantibodywastestedbyHIassayfor10successiveweeks.Theclinicalsymptomswereinvestigatedandvinlsdetectiontestswereped'o瑚ed.【Result】Theresultsshowedantibodytitersofpigsimmunizedwithmoderatedose(4mLperpig)andhighdose(6mLpe
8、rpig)werehigherthanthatofthelowdose(2mLperpig).ThebodytemperaturesofpigsbetweenimmunizedgroupandcontIDlgroupdisplayednosigni6cantdifkIences.Pigsoftheimmunizedgrouphadnoobviousclinicalsymptoms,indicatingthatpigswereweupmtectedbyvaccine.【conclusion】Ina