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1、中國中醫(yī)急癥2014年1月第23卷第1期JETCM.Jan.2014,V01.23,N0.1·25··研究報告·黃芪多糖對免疫性肝損傷小鼠免疫調(diào)節(jié)的影響術(shù)李天一汪麗佩吳國琳2(1.浙江中醫(yī)藥大學(xué),浙江杭州310053;2.浙江大學(xué)醫(yī)學(xué)院附屬第一醫(yī)院,浙江杭州310013)中圖分類號:R285.5文獻標(biāo)志碼:A文章編號:1004—745X(2014)01—0025—03doi:10.3969~.issn.1004-745X.2014.01.012【摘要】目的觀察不同劑量的黃芪多糖對免疫性肝損傷小鼠的肝功能、骨橋蛋白(OPN)、腫瘤壞死因子
2、一(TNF一)的影響,初步探討黃芪多糖治療病毒性肝炎的作用機制,為其在臨床上的應(yīng)用提供理論依據(jù)。方法各組小鼠給予不同因素干預(yù),造模結(jié)束后各組小鼠摘眼球采血,脫椎處死取肝臟。檢測血清中丙氨酸氨基轉(zhuǎn)移酶(A)、天冬氨酸氨基轉(zhuǎn)移酶(AST),ELISA檢測OPN、TNF一0【,免疫組織化學(xué)法檢測肝臟中OPN、TNF—ft.蛋白的表達。結(jié)果黃芪多糖可降低血清中的AIJT、AST(P<0.05或0.01),高劑量黃芪多糖使OPN、TNF一僅水平顯著降低(P<0.05),OPN與TNF一僅呈正相關(guān)。結(jié)論黃芪多糖對免疫性肝損傷模型具有一定的保護肝臟和
3、免疫調(diào)節(jié)作用,可減輕炎癥和肝細胞損傷、改善肝功能?!娟P(guān)鍵詞】黃芪多糖免疫性肝損傷免疫調(diào)節(jié)骨橋蛋白腫瘤壞死因子一StudyonOsteopontinofAstragalusPolysaccharidesOilMicewithImmunologicalLiverInjuryLITian-yi,WANGLi-pei,Guo-lin2.1ZhejiangChineseMedicalUniversity,Zhejiang,Hangzhou310053,China;2TheFirstAfiliatedHospitalofMedicdSchoolof
4、ZhejiangUnwe~ity,Zhejiang,Hangzhou310003,China【Abstract】Objective:Toobservetheeffectofastragaluspolysaccharides(APS)onliverfunction,osteopontin(OPN),tumornecrosisfactor-or(TNF-a)ofmicewithimmunologicalliverinjuryinordertoexplorethethera—peutiemechanismofAPSintreatinghepa
5、titisandofferexperimentalevidencetoitsclinicalapplication.Meth-ods:40ratsweredividedrandomlyinto5groups.Aftermodelingandweighingbloodwerecollecedbyexcisingtheeyeballs,liver,spleenandthymusweretakentocalculateorgansindex.Theserumwasseparatedtodeterminealanineaminotransfer
6、ase(ALT),aspartateaminotransferase(AST),OPNandTNF一0【.Thespecimensofleftlobeofliverin10%formaldehydewasusedtomakepathologicalsliceforobservingliverpathology.Theseindicatorsweredeterminedbyimmunohisto—chemistrymethodincludingexpressionofOPNandTNF—protein.Reslllts:APSdecrea
7、sedtheALT,ASTinserum(P<0.05or0.O1).HighdosageofAPSreducedthe1evelsofOPNandTNF—OtsignificantlyfJP<0.05).OPNandTNF-awerepositivelycorrelated.Conclusion:APShasthecertainprotectionandimmunoregulativeefectonimmunologicalliverinjury,reducingliverinflammationandlivercellinjury.
8、Itcanimproveliverfunctionofexperimentalimmunologicalliverinjuryinmice.【Keywords】Astragaluspolysaccharid