燈盞花素減輕順鉑對(duì)大鼠腎損害及腎細(xì)胞凋亡機(jī)制的研究.pdf

《燈盞花素減輕順鉑對(duì)大鼠腎損害及腎細(xì)胞凋亡機(jī)制的研究.pdf》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在應(yīng)用文檔-天天文庫。

1、中華實(shí)用診斷與治療雜志2014年3月第28卷第3期JournalofChinesePracticalDiagnosisandTherapy·251··論著·燈盞花素減輕順鉑對(duì)大鼠腎損害及腎細(xì)胞凋亡機(jī)制的研究馬天江，張磊，張智慧，劉娜，任亮，張印坡，馮學(xué)召。(1．漯河醫(yī)學(xué)高等?？茖W(xué)校第一附屬醫(yī)院腫瘤科，河南漯河462000；2．漯河醫(yī)學(xué)高等?？茖W(xué)?；A(chǔ)部藥理教研室，河南漯河462000)摘要：目的探討應(yīng)用燈盞花素減輕順鉑對(duì)大鼠腎損害及腎細(xì)胞凋亡的作用機(jī)制。方法48只SD大鼠分為對(duì)照組、模型組、25mg／kg燈盞花素組(燈盞花素1組)和50mg／kg燈盞花素

2、組(燈盞花素2組)各12只。對(duì)照組給予羧甲基纖維素鈉溶液灌胃，模型組、燈盞花素1組和2組均腹腔注射順鉑8mg／kg造模。燈盞花素1組和2組分別以25mg／kg和50mg／kg燈盞花素灌胃，給藥7d后計(jì)算4組腎臟指數(shù)，采用原位缺口末端標(biāo)記法檢測(cè)腎細(xì)胞凋亡情況，左腎采用蛋白質(zhì)印跡法檢測(cè)腎皮質(zhì)Bax、Bcl一2表達(dá)水平，右腎采用免疫組織化學(xué)法檢測(cè)腎組織凋亡蛋白Bax和Bcl一2表達(dá)水平。結(jié)果模型組、燈盞花素1組和2組腎臟指數(shù)、凋亡指數(shù)及Bax、Bcl一2表達(dá)水平高于對(duì)照組(P

3、于模型組、Bcl-2表達(dá)水平高于模型組(P<0．05)；燈盞花素1組腎臟指數(shù)和凋亡指數(shù)高于2組(P<0．05)。結(jié)論燈盞花素可增強(qiáng)凋亡蛋白Bcl-2表達(dá)、降低Bax表達(dá)，影響線粒體凋亡通路，從而減輕順鉑對(duì)腎損害大鼠腎細(xì)胞的凋亡。關(guān)鍵詞：腎損害；大鼠；燈盞花素；順鉑；細(xì)胞凋亡MechanismofBreviscapineinreducingcisplatin‘inducedkidneydamageandapoptosisofkidneycellsinratsMATian-jiang，ZHANGLei，ZHANGZhi—hui，LIUNa，RENLiang，

4、ZHANGYin-po，F(xiàn)ENGXue-zhao(*DepartmentofOncology，theFirstAffiliatedHospitalofLuoheMedicalCollege，Luohe462000，China)Abstract：ObjectiveToexplorethemechanismofBreviscapineinreducingcispIatin—inducedkidneydamageandapoptosisofkidneycells．MethodsForty-eightSDratswererandomlydividedintoco

5、ntrolgroup，modelgroup，25mg／kgBreviscapinegroup(Breviscapine1group)and50mg／kgBreviscapinegroup(Breviscapine2group)，with12ratsineachgroup．Controlgroupwasgivenlavagewithearboxymethylcellulosesodium，andtheotherthreegroupswereestablishedmodelsbyintraperitonea1injectionof8mg／kgcisplati

6、n，followedbylavagewith25mg／kgand50mg／kgBreviscapinerespectivelyinBreviscapine1and2groups．Fourdayslater，thekidneyindexeswerecalculated．TheapoptosisindexesofkidneycellsweredetectedbyTUNELmethod．TheexpressionsofBaxandBcl一2inkidneycellsweredetectedbyWesternblotmethodintheleftkidney，a

7、ndtheexpressionsofBaxandBcl一2weredetectedbyimmunohistochemiealmethodintherightkidney．ResultsThekidneyindexes，apoptosisindexes，andBaxandBel一2levelswerehigherinmodelgroupandBreviscapinegroupsthanthoseincontrolgroup(P

8、ower，andBcl一2levelwashigherinBreviscapin