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《神經(jīng)生長因子及其受體在缺血缺氧性腦損傷中的作用機(jī)制》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在應(yīng)用文檔-天天文庫。
1、目錄一、中文摘要········································I二、英文摘要········································II三、引言·········································1四、正文材料與方法········································3結(jié)果············································5討論··················
2、······················10結(jié)論········································20參考文獻(xiàn)········································21五、綜述正文·········································24參考文獻(xiàn)··········································29六、后記········································33中文摘
3、要目的：通過對(duì)大鼠缺血缺氧性腦損傷模型注射不等含量的神經(jīng)生長因子，并和正常大鼠模型的不同部位神經(jīng)生長因子濃度和受體含量進(jìn)行對(duì)比，得出神經(jīng)生長因子和其受體在缺血缺氧性腦損傷中的作用機(jī)制，為臨床指導(dǎo)治療缺血缺氧性腦損傷提供依據(jù)。方法：1.大鼠模型資料：選取南方醫(yī)科大學(xué)動(dòng)物中心的16-18周大鼠若干只，雌雄不限，模型均隨機(jī)篩選，統(tǒng)計(jì)結(jié)果無統(tǒng)計(jì)學(xué)差異。2.方法：將這些隨機(jī)抽取的大鼠模型一共50只，按照是否手術(shù)和手術(shù)注射神經(jīng)生長因子的情況的不同分為5組，A組的大鼠模型正常喂養(yǎng)，B組的大鼠模型做左頸動(dòng)脈結(jié)扎手術(shù)，并且在持續(xù)喂養(yǎng)
4、時(shí)每日注射生理鹽水；C組的大鼠模型做左頸動(dòng)脈結(jié)扎手術(shù)，并且在持續(xù)喂養(yǎng)時(shí)每日注射鼠神經(jīng)生長因子30ug；D組的大鼠模型做左頸動(dòng)脈結(jié)扎手術(shù)，并且在持續(xù)喂養(yǎng)時(shí)每日注射鼠神經(jīng)生長因子60ug；E組的大鼠模型做左頸動(dòng)脈結(jié)扎手術(shù)，并且在持續(xù)喂養(yǎng)時(shí)每日注射鼠神經(jīng)生長因子90ug，比較這五組大鼠模型在術(shù)后1天、術(shù)后一周、術(shù)后兩周、術(shù)后四周這幾個(gè)時(shí)間段頭部、腹部和尾部不同部位神經(jīng)生長因子濃度和TrkB受體含量的變化情況。3.數(shù)據(jù)分析：各組大鼠模型的基本資料情況經(jīng)正態(tài)性檢查，服從正態(tài)分布，且各組間無統(tǒng)計(jì)學(xué)差異。使用SAS6.12統(tǒng)計(jì)學(xué)
5、軟件按照2*2析因設(shè)計(jì)進(jìn)行統(tǒng)計(jì)學(xué)分析，分別通過對(duì)大鼠模型術(shù)后的統(tǒng)計(jì)指標(biāo)結(jié)果做出評(píng)估。數(shù)值型變量以均數(shù)±標(biāo)準(zhǔn)差表示。結(jié)果：五組大鼠模型在術(shù)后相同時(shí)間內(nèi)同組不同部位的神經(jīng)生長因子濃度和TrkB受體含量的統(tǒng)計(jì)學(xué)差異具有統(tǒng)計(jì)學(xué)意義（P＜0.05）；在術(shù)后相同時(shí)間內(nèi)不同組別同部位的神經(jīng)生長因子濃度和TrkB受體含量的統(tǒng)計(jì)學(xué)差異具有統(tǒng)計(jì)學(xué)意義（P＜0.05）；在術(shù)后不同時(shí)間內(nèi)同組別同部位的神經(jīng)生長因子濃度和TrkB受體含量的統(tǒng)計(jì)學(xué)差異具有統(tǒng)計(jì)學(xué)意義（P＜0.05）。關(guān)鍵詞：神經(jīng)生長因子；TrkB受體；缺血缺氧性腦損傷；作用機(jī)制
6、AbstractsObjective:Basedontheratswithhypoxiaischemiabraindamagemodelofinjectionunequalcontentofnervegrowthfactorandnerveindifferentpartsofthemodelofnormalratgrowthfactorconcentrationandreceptorcontentcomparison,drawsthemechanismofnervegrowthfactoranditsrecepto
7、rinhypoxicischemicbraindamageintheclinicalguidanceforprovidethebasisforthetreatmentofischemiaandhypoxiainjurycerebral.Methods:1.Datafromratsmodel:selectionofanimalcenterofSouthernMedicalUniversity16-18weeksseveralbigrats,maleorfemale,themodelswererandomlyselec
8、ted,nostatisticaldifferenceinresults.2.Methods:theratmodeloftherandomsampleof50intotal,accordingtothedifferentoperationandoperationwhetherinjectionofnervegrowthfactorconditionsinto
