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1、武漢植物學(xué)研究2006,24(3):235~239JournalofWuhanBotanicalResearch不同貯藏溫度對枇杷果肉木質(zhì)化及相關(guān)酶活性的影響*吳錦程,唐朝暉,陳群,夏海琳,賴育蘭(福建省莆田學(xué)院環(huán)境與生命科學(xué)系,福建莆田351100)摘要:將解放鐘枇杷(EriobotryajaponicaLind.lcv.Jiefangzhong)果實于4和12下貯藏,研究了不同貯藏溫度對采后枇杷果肉木質(zhì)化及相關(guān)酶活性的影響。結(jié)果表明,4貯藏的枇杷果肉苯丙氨酸解氨酶(phenylalanin
2、eammonialyase,PAL)、4-香豆酸輔酶A連接酶(4-coumaratecoenzymeAligase,4-CL)、肉桂醇脫氫酶(cinnamylalco-holdehydrogenase,CAD)和過氧化物酶(peroxidase,POD)活性高于12貯果,而多酚氧化酶(polyphenoloxidase,PPO)活性低于12貯果。4貯藏的枇杷果肉硬度和木質(zhì)素含量不斷增加,果肉木質(zhì)化較為嚴(yán)重;12貯藏的枇杷果肉硬度和木質(zhì)素含量低于4貯果,果肉木質(zhì)化程度較低。低溫誘導(dǎo)PAL和POD活性
3、的上升是導(dǎo)致枇杷果肉發(fā)生木質(zhì)化的主要原因。關(guān)鍵詞:枇杷;果肉木質(zhì)化;貯藏溫度;苯丙氨酸解氨酶(PAL);過氧化物酶(POD)中圖分類號:S667.3;Q946.5文獻(xiàn)標(biāo)識碼:A文章編號:1000-470X(2006)03-0235-05TheEffectsofDifferentStorageTemperaturesonLignificationandRelatedEnzymesActivitiesinLoquatPulp*WUJin-Cheng,TANGChao-Hu,iCHENQun,
4、XIAHa-iLin,LAIYu-Lan(EnvironmentandLifeScienceDepartment,PutianUniversity,Putian,Fujian351100,China)Abstract:Theeffectsofdifferentstoragetemperaturesonlignificationandrelatedenzymeactivitiesoflo-quat(EriobotryajaponicaLind.lcv.Jiefangzhong)pulpstoredat4a
5、nd12werestudied.Theresultsshowedthatphenylalanineammonialyase(PAL,EC4.1.3.5.)activity,4-coumaratecoenzymeAligase(4-CL,EC6.2.1.12.)activity,cinnamylalcoholdehydrogenase(CAD,EC1.1.1.195.)activityandperoxidase(POD,EC1.11.1.7.)activityofloquatpulpstoredat4we
6、rehigherthanthosestoredat12,whilepolyphenoloxidase(PPO,EC1.10.3.2.)activitywaslower.Thefirmnessandlignincon-tentofloquatpulpat4increasedduringstorage,whichresultsincomparativelyseriouslignificationinloquatpulp.Thefirmnessandlignincontentofloquatpulpat12
7、werelowerthanat4,hencealo-werleveloflignificationinloquatpulp.Themainfactorforlignificationinloquatpulpisthatthelowtemperaturecausedhigherphenylalanineammonialyaseactivityandperoxidaseactivity.Keywords:Loqua;tLignificationofpulp;Storagetemperature;Phenyla
8、lanineammonialysae;Perox-idase[2]枇杷(EriobotryajaponicaLind.l)為原產(chǎn)中國的異常所致。本研究主要從植物苯丙烷代謝途徑一種亞熱帶保健水果,果實皮薄多汁,極易被碰傷或來探討相關(guān)酶活性變化與冷藏枇杷果肉木質(zhì)化的關(guān)受微生物侵染而腐爛,不耐貯運(yùn),鮮食供應(yīng)期短,外系,以期為控制冷藏枇杷果肉木質(zhì)化發(fā)生提供理論[1]銷就更為困難,制約了枇杷種植效益的提高。目依據(jù)。前枇
