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1、(中文標(biāo)題:黑體,二號)枸杞甜菜堿含量測定方法的比較研究ComparativestudyonmethodsfordeterminationofbetainefromLyciumbarbarumL.(英文標(biāo)題:TimesNewRoman,20)院系:生命科學(xué)學(xué)院專業(yè):生物技術(shù)年級:2003級學(xué)號:12003242527姓名:馬佳卉指導(dǎo)教師:張自萍(黑體,三號)14枸杞甜菜堿含量測定方法的比較研究(標(biāo)題:黑體二號)摘要(黑體,小四):本實驗利用甲醇加熱回流提取枸杞甜菜堿,提取物經(jīng)活性炭脫色、雷氏鹽沉淀、分光光度法測定不同
2、產(chǎn)地枸杞甜菜堿含量;或直接利用NH2柱未經(jīng)衍生高效液相色譜法測定枸杞甜菜堿含量。通過比較分光光度法和高效液相色譜法測定的枸杞甜菜堿含量,選擇適宜的甜菜堿含量測定方法。由實驗結(jié)果可知,NH2柱未經(jīng)衍生高效液相色譜法快速、簡便、重復(fù)性好,所測甜菜堿含量明顯高于分光光度法。本研究表明NH2柱未經(jīng)衍生高效液相色譜法能快速準(zhǔn)確地測定枸杞甜菜堿含量,可用于枸杞的質(zhì)量控制.(仿宋,小四,1.5倍行距)關(guān)鍵詞:枸杞子;甜菜堿;分光光度法;高效液相色譜法Comparativestudyonmethodsfordetermination
3、ofbetainefromLyciumbarbarumL.(TimesNewRoman,18,加粗)Abstract(TimesNewRoman,14,加粗):Toapplymethanol-refluxedextractionmethodtoextractbetainefromLyciumbarbarumL.,extractionwasdecoloredbyactivecarbon,precipitatedwithRemeckesaltandthecontentsofbetainefromdifferentprod
4、ucingareasweredeterminedbyspectrophotometry;andthecontentsofbetaineweredirectlydeterminedbyHPLCwithNH2stationaryphasewithoutderivatizationofbetaine.ThesuitabledeterminationmethodwasselectedthroughcomparingthemethodsofspectrophotometryandHPLCindeterminingthecont
5、entofbetainefromLyciumbarbarumL.FromtheresultwecanseethatHPLCisrapid,accurate,andthecontentsofbetainedeterminedbyHPLCwerehigherthanspectrophotometry.ThisstudyshowedthatHPLCwithNH2stationaryphasewithoutderivatizationofbetainecanbeappliedtodeterminethecontentofbe
6、tainefromLyciumbarbarumL.rapidlyandaccurately.(TimesNewRoman,12,1.5倍行距)Keywords:LyciumbarbarumL;betaine;spectrophotometry;HPLC14目錄(黑體,三號)前言………………………………………………………………………………………41.材料與器材………………………………………………………………………………51.1原料……………………………………………………………………………………51.2試劑…………………
7、…………………………………………………………………61.3儀器與設(shè)備……………………………………………………………………………62.實驗方法………………………………………………………………………………62.1分光光度法測定枸杞甜菜堿含量……………………………………………………62.1.1實驗原理……………………………………………………………………………62.1.2枸杞甜菜堿供試品溶液的制備……………………………………………………62.1.3甜菜堿標(biāo)準(zhǔn)曲線的建立……………………………………………………………62.1.4
8、供試品溶液的含量測定……………………………………………………………72.2高效液相色譜法測定枸杞甜菜堿含量………………………………………………72.2.1色譜分析條件………………………………………………………………………82.2.2枸杞甜菜堿供試品溶液的制備……………………………………………………82.2.3對照品溶液的制備…………………………