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1、中國科技論文在線http://www.paper.edu.cn參芪解郁方對產(chǎn)后抑郁大鼠Treg/Th17平#衡的干預(yù)作用研究1211**曲淼，李凈婭，趙瑞珍，唐啟盛5（1.北京中醫(yī)藥大學(xué)第三附屬醫(yī)院,北京100029；2.浙江省中醫(yī)院,浙江杭州310006）摘要：目的：觀察產(chǎn)后抑郁癥模型大鼠Treg/Th17細胞比例的變化，闡明參芪解郁方對Treg/Th17平衡的干預(yù)作用。方法：SD雌性大鼠隨機分為正常組、假手術(shù)、模型組、中藥組10和西藥組，分設(shè)灌胃1、2、4周三個時點。采用流式細胞術(shù)檢測三個時點各組大鼠外周血Treg細胞和Th17細胞比例，并計算Treg
2、/Th17比值。結(jié)果：1w、2w模型組Treg/Th17較正常組明顯升高（P<0.01），而4w模型組Treg/Th17較正常組明顯降低（P<0.01），4w中藥組較模型組升高明顯（P<0.01）。結(jié)論：參芪解郁方可通過調(diào)控Treg/Th17的失衡狀態(tài)發(fā)揮抗抑郁作用。15關(guān)鍵詞：產(chǎn)后抑郁癥；參芪解郁方；Treg/Th17平衡中圖分類號：R285.5ImmunedysregulationonregulatoryTcellsandTh17cellsinpostpartumdepressionandtherolesof20Shen-Qi-Jie-Yu-Fang1
3、211QuMiao,LiJingya,ZhaoRuizhen,TangQisheng(1.ThirdaffiliatedhospitalofBeijingUniversityofChineseMedicine,Beijing100029;2.ZhejiangProvicialHospitalofTCM,Hangzhou310006)Abstract:IObjective:Shen-Qi-Jie-Yu-Fang(SJFang),establishedbyourteamleaderDr.Tang,was25oneoftheusefultherapyofpost
4、partumdepression(PPD)inTraditionalChineseMedicine(TCM).SJFangwastonifyingdeficiency,removingbloodstasis,nourishingbrainandinducingresuscitation.However,theimpactofimmunesystemofSJFangonPPDwasstillunknown.So,thepresentstudywasdesignedtoexploretheimmunomodulationeffectofSJFangonregu
5、latoryTcellsandTh17cells.MaterialsandMethods:NinetyFemaleSprague-Dawleyratsweredividedintofivegroupsrandomly.30PPDratsweremodelingwithhormonesimulatedpregnancy(HSP).RatsinPPDgroupwereovariecImmunedysregulationonregulatoryTcellsandTh17cellsinpostpartumdepressionandtherolesofShen-Qi
6、-Jie-Yu-Fangtomizedbilaterally,butratsinshamgroupwereexteriorizedwithassociatedfatpadnearovary.Theninjectionofprogesteroneandestradiolbenzoatewasadministeredfor23days.Onday24,theinjectionswereterminatedtoinitiatehormonewithdrawal.AfterHSP35modelprocedure,ratsweregavageadministrate
7、dfor1week,2weeksand4weeks.Immediately,behavioralassessmentsofsucroseconsumptionandforcedswimtest(FST)wereconducted.ImmuneimbalancestatuswasobservedinanalyzinglevelsofregulatoryTcellsandTh17cellsinperipheralbloodmononuclearcellswithflowcytometry.Results:TheresultssuggestedthatTregc
8、ellsdepletionmodulatedalterations
