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1、棉花學報CottonScience2014袁26淵6冤院499~505利用染色體片段代換系定位棉花抗黃萎病QTL1111*2*趙君袁劉劍光袁吳巧娟袁肖松華袁袁有祿淵1.江蘇省農(nóng)業(yè)科學院經(jīng)濟作物研究所/農(nóng)業(yè)部長江下游棉花和油菜重點實驗室袁江蘇南京210014曰2.中國農(nóng)業(yè)科學院棉花研究所/棉花生物學國家重點實驗室袁河南安陽455000冤摘要院通過陸地棉與海島棉雜交并與陸地棉回交袁獲得1個染色體片段代換系蘇VR043袁抗江蘇非落葉型黃萎病菌系BP2遙分子檢測表明袁蘇VR043兼有陸地棉蘇棉8號的遺傳背景和海7124的D4染色體片段遙
2、以蘇VR043和蘇棉8號為親本構(gòu)建包含176個單株的F2群體袁通過標記分析和F2:3家系抗病鑒定袁發(fā)現(xiàn)抗病性狀與標記NAU3392連鎖袁值為2.3伊10-12遙根據(jù)棉花D組染色體測序結(jié)果袁參照置換區(qū)段的基因組序列袁合成92對SSR引物曰PCR擴增分析發(fā)現(xiàn)袁有5對引物在蘇棉8號和蘇VR043之間表現(xiàn)多態(tài)性袁并將抗病主效QTL定位在標記ZHX32和NAU3392之間袁標記間遺傳距離為3.2cM袁QTL解釋表型變異64.8%遙同時參照棉花D組測序結(jié)果袁提取對應的置換區(qū)段序列袁預測包含63個基因袁基因聚類分析表明7個基因參與抗逆反應袁其
3、中1個基因與抗病相關(guān)遙關(guān)鍵詞院棉花曰黃萎病曰分子標記曰抗病基因曰QTL中圖分類號院S562.03文獻標志碼院A文章編號院1002-7807(2014)06-0499-071111*2*ZhaoJun,LiuJianguang,WuQiaojuan,XiaoSonghua,YuanYoulu(1.210014,;2.455000,)TodissecttheVerticilliumwiltresistanceQTLandfindmolecularmarkerscloselylinkedwiththeresistancetrait,w
4、ecreatedanewmaterial.BycrossingL.cv.Sumian8astherecipientparentandL.cv.Hai7124asthedonorparent,wegeneratedachromosomesegmentintrogressionline(CSIL),SuVR043,withhigherresis-tancetonon-defoliatingBP2isolatesof.SuVRo43wascharacterizedascarryingonehomozygouschromo-some22
5、(D4)segmentrepresentedbytheSSRmarkerNAU3392fromcv.Hai7124inthebackgroundoftheup-landcottonSumian8.WeconstructedanF2mappingpopulation,SuVR043伊Sumian8,containing176individuals.BasedonSSRmarkersandFphenotypesforresistancetowiltstrainBP2,singlemarkeranalysisshowedtheresi
6、stancephe-學報頁腳2:3notypewaslinkedtothemakerNAU3392withavalueof2.3伊10-12.UsingtherecentlyreleasedcottonDgenomesequencein-formationasareference,weextractedtheintrogressionsegmentsequenceanddesigned92SSRprimerpairs,fromwhichfivepolymorphicSSRlociweredetectedbetweenSuVR04
7、3andSumian8andusedtomaptheresistanceQTLontheintrogressionsegment.TheQTLwasanchoredbetweentheZHX32andNAU3392lociwithadistanceof3.2cM,andex-plained64.8%ofthephenotypicvariation.Moreover,wepredicted63candidategenesusingthecottonDgenomesequencein-formation.TheresultsofGe
8、neOntologyanalysisindicatedthatthesegeneswereinvolvedindifferentbiologicalprocesses,withsevenofthemparticipatinginthestresssignaltr
