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《國內(nèi)外蠶豆核心種質(zhì)ssr遺傳多樣性對比及微核心種質(zhì)構(gòu)建》由會員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、作物學(xué)報(bào)ACTAAGRONOMICASINICA2014,40(7):1311?1319http://zwxb.chinacrops.org/ISSN0496-3490;CODENTSHPA9E-mail:xbzw@chinajournal.net.cnDOI:10.3724/SP.J.1006.2014.01311國內(nèi)外蠶豆核心種質(zhì)SSR遺傳多樣性對比及微核心種質(zhì)構(gòu)建1111211,*姜俊燁楊濤王芳方俐仲偉文關(guān)建平宗緒曉12中國農(nóng)業(yè)科學(xué)院作物科學(xué)研究所/農(nóng)作物基因資源與基因改良國家重大科學(xué)工程,北京100081;
2、甘肅農(nóng)業(yè)大學(xué)生命科學(xué)技術(shù)學(xué)院,甘肅蘭州730070摘要:運(yùn)用24對SSR引物,對國內(nèi)外1075份初級地理蠶豆核心種質(zhì)的遺傳多樣性分析顯示,等位變異數(shù)、有效等位變異數(shù)及Shannon’s信息指數(shù)分別為8.54、2.26和1.02;對全部參試資源進(jìn)行聚類分析,沒有發(fā)現(xiàn)明顯的群體結(jié)構(gòu),表明初級地理核心種質(zhì)的代表性較好,遺傳背景較廣泛。之后采用每個(gè)類內(nèi)隨機(jī)抽樣的方法構(gòu)建含有129份國內(nèi)資源和63份國外資源的蠶豆微核心種質(zhì),等位基因變異數(shù)、有效等位變異數(shù)和Shannon’s信息指數(shù)的保留比例分別為87.32%、101.26%
3、和101.82%;經(jīng)t檢驗(yàn)得出微核心種質(zhì)與全部參試資源群體間遺傳多樣性差異不顯著,表明構(gòu)建的微核心種質(zhì)的遺傳多樣性可以代表初級地理蠶豆核心種質(zhì)。關(guān)鍵詞:SSR;蠶豆;遺傳多樣性;核心種質(zhì);微核心種質(zhì)GeneticDiversityAnalysisofGermplasmResourcesandConstructionofMini-coreCollectionsforViciafabaL.atHomeandAbroad111121JIANGJun-Ye,YANGTao,WANGFang,FANGLi,ZHONGWei-
4、Wen,GUANJian-Ping,andZONG1,*Xu-Xiao1NationalKeyFacilityforCropGeneResourcesandGeneticImprovement/InstituteofCropScience,ChineseAcademyofAgriculturalSciences,2Beijing100081,China;CollegeofLifeScienceandTechnology,GansuAgriculturalUniversity,Lanzhou730070,ChinaA
5、bstract:Thegeneticdiversityof1075genotypesfromaprimarygeographiccorecollectionoffababean(ViciafabaL.)wasanalyzedbyusingSSRmarkers.Thenumberofobservedandeffectiveallelesof8.54and2.26,theShannon’sinformationindexof1.02,weredetected.Unconspicuouspopulationstructu
6、reamongthe1075genotypesindicatedanextensivegeneticbackgroundandnicerepresentativeness.Amini-corecollectioncomprised129Chineseand63overseafababeangenotypeswasrandomlysampledfromeachclusterofdendrogramincludingallthe1075testedgenotypes.Theobservedalleles,effecti
7、veallelesandShannon’sinformationindexofthemini-corecollectioncovered87.32%,101.26%,and101.82%oftheprimarygeographiccorecollectionrespectively.Thet-testdidnotshowsignificantdifferencesingeneticdiversitybetweentheprimarygeographiccorecollectionandthemini-corecol
8、lection,whichvalidatedtherepresentativenessofthemini-corecollectioningeneticdiversityoffababean.Themini-corecollectionwillplayanimportantroleinexplorationofnewgeneswithinfababean.K
