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《大豆鹽脅迫相關(guān)gmnac基因的鑒定、表達及變異分析》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、作物學(xué)報ACTAAGRONOMICASINICA2016,42(7):990?999http://zwxb.chinacrops.org/ISSN0496-3490;CODENTSHPA9E-mail:xbzw@chinajournal.net.cnDOI:10.3724/SP.J.1006.2016.00990大豆鹽脅迫相關(guān)GmNAC基因的鑒定、表達及變異分析1,211111,?張彥威張禮鳳李偉王彩潔張軍徐冉12山東省農(nóng)業(yè)科學(xué)院作物研究所,山東濟南250131;東北農(nóng)業(yè)大學(xué)大豆生物學(xué)教育部重點實驗室,黑龍江哈爾濱150001摘要:NAC基因在植物的逆境脅迫
2、中發(fā)揮著重要作用。本研究參照水稻和擬南芥的逆境相關(guān)NAC基因,采用生物信息學(xué)方法鑒定了大豆逆境相關(guān)GmNAC基因,利用熒光定量PCR技術(shù)分析了GmNAC基因在耐鹽差異的大豆品種根部、葉片的表達及其對NaCl脅迫的應(yīng)答,采用反轉(zhuǎn)錄PCR技術(shù)克隆了表達差異顯著的GmNAC基因。結(jié)果表明,大豆GmNAC基因家族包含175個基因,其中11個基因所編碼的GmNAC蛋白與水稻和擬南芥的逆境相關(guān)NAC蛋白位于同一進化分支,這些蛋白具有高度保守的NAC結(jié)構(gòu)域;這11個GmNAC基因在大豆根部的表達均高于在葉片,而且在葉片和根部均受NaCl誘導(dǎo),部分基因在根部和葉片以及品種間
3、表現(xiàn)出不同的表達規(guī)律;在大豆品種齊黃34、徐豆10和汾豆95中,Glyma06g11970.1存在3個同義突變和1個非同義突變,Glyma06g16440.2存在1個同義突變。關(guān)鍵詞:大豆;GmNAC;進化樹;NaCl處理;表達分析;序列變異Identification,ExpressionandVariationAnalysisofSaltToleranceRelatedGmNACGenesinSoybean1,211111,?ZHANGYan-Wei,ZHANGLi-Feng,LIWei,WANGCai-Jie,ZHANGJun,andXURan12Cr
4、opResearchInstitute,ShandongAcademyofAgriculturalSciences,Jinan250131,China;KeyLaboratoryofSoybeanBiologyofMinistryofEducation,NortheastAgriculturalUniversity,Harbin150001,ChinaAbstract:NACgenesplayanimportantroleinplantstresstolerance.Inthisstudy,bioinformaticmethodwasusedtoidenti
5、fythestressrelatedGmNACgeneinsoybean.TheexpressionofcandidatedGmNACgenesinrootandleafwasanalyzedinsoybeanwithNaCltreatmentbyReal-timePCR.ReversetranscriptionPCRwasperformedtoclonegeneswithsignificantdifferenceinexpression.Theresultsshowedthattherewere175genesinsoybeanGmNACgenefamil
6、y.Therewere11GmNACproteinswithhighlyconservedNAClocatedonthesameevolutionarybranchwiththestressrelatedNACproteinsinriceandArabidopsis.Theexpressionof11GmNACgenesinsoybeanrootwashigherthanthatinleaf.TheGmNACgeneswereallinducedbyNaClstress,butpartoftheGmNACgenesshoweddifferentexpress
7、ionlevelsbetweenrootandleafinsoybeanvarietieswithdifferentsalttolerances.Therewerethreesynonymousmutationsandonenon-synonymousmutationontheCDSregionofGlyma06g11970.1andonesynonymousmutationontheCDSregionofGlyma06g16440.2inQihuang34,Xudou10,andFendou95.Keywords:Soybean;GmNAC;Phyloge
8、netictree;NaCltreatment;Ex
