棉花抗黃萎病鑒定與評價(jià)標(biāo)準(zhǔn)的商榷

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1、棉花學(xué)報(bào)CottonScience2017,29(1):50―58棉花抗黃萎病鑒定與評價(jià)標(biāo)準(zhǔn)的商榷*****趙麗紅袁馮自力袁李志芳袁馮鴻杰袁師勇強(qiáng)袁張蕓袁朱荷琴淵棉花生物學(xué)國家重點(diǎn)實(shí)驗(yàn)室/中國農(nóng)業(yè)科學(xué)院棉花研究所袁河南安陽455000冤摘要院揖目的銥通過系統(tǒng)研究黃萎病抗病鑒定的關(guān)鍵技術(shù)和評價(jià)標(biāo)準(zhǔn)袁解決現(xiàn)有棉花品種黃萎病抗性評價(jià)標(biāo)準(zhǔn)中存在的問題袁建立新的田間棉花黃萎病分級標(biāo)準(zhǔn)和評價(jià)標(biāo)準(zhǔn)遙揖方法銥以14個(gè)2014年國家主推棉花品種為試驗(yàn)材料袁從接種量堯可靠樣本量堯病情分級和評價(jià)方法4個(gè)方面系統(tǒng)研究黃萎病抗性鑒定的關(guān)鍵技術(shù)和評價(jià)標(biāo)準(zhǔn)遙揖結(jié)果銥確定了小區(qū)接種和行溝接種的最

2、佳接菌量分別為60g窯m－2和10g窯m－1袁小區(qū)調(diào)查最低樣本量為50株曰制定了新的分級標(biāo)準(zhǔn)為0級淵0%冤堯1級淵1%～33%冤堯2級淵34%～66%冤堯3級淵67%～99%冤和4級淵100%冤遙連續(xù)2年利用相對病程發(fā)病面積和發(fā)病高峰期的相對病情指數(shù)2種評價(jià)方法判定品種抗病性袁前者作為抗病性評價(jià)的依據(jù)更為科學(xué)可靠袁一致率達(dá)到100%遙揖結(jié)論銥本試驗(yàn)為完善抗病鑒定技術(shù)操作規(guī)程袁規(guī)范抗性評價(jià)方法袁促進(jìn)棉花抗病品種的選育堯?qū)彾ê屯茝V提供了科學(xué)依據(jù)遙關(guān)鍵詞院黃萎病曰接種技術(shù)曰最低樣本量曰分級標(biāo)準(zhǔn)曰抗性評價(jià)方法中圖分類號院S435.621文獻(xiàn)標(biāo)志碼院A文章編號院1002-7

3、807淵2017冤01-0050-09院10.11963/issn.1002-7807.201701006*****ZhaoLihong,FengZili,LiZhifang,FengHongjie,ShiYongqiang,ZhangYun,ZhuHeqin(455000,)[Objective]ThepurposeofthisinvestigationwastosystematicallystudythestandardtechniquesusedtoidentifyandevaluateVerticilliumwiltresistanceincottonto

4、solvethetraditionalmethodsordevelopanewone.[Method]Fourteennational-lyrecommendedcottonvarietiesin2014wereusedtosystematicallystudytheimportanceofinoculumconcentration,samplenumber,diseaselevel,andevaluationmethod.Wealsousedtherelativeareaunderthediseaseprogresscurveandtherelateddisea

5、seindexatthediseasepeaktoevaluateVerticilliumwiltresistanceincottonintwoconsecutiveyears.[Results]Theidealconcentrationsforplotandcolumninoculationswere60g窯m-2and10g窯m-1basedonthereal-timemonitoringofthediseasein-dexofsusceptiblevariety(Jimian11)plants.Toimprovethereliabilityofthedata

6、,theminimumnumberofinvestigatedplantswassetas50accordingtothedivergencerateanalysis.Thenewdiseasegradingstandardrelatedtocottonyieldwasbasedonthefoliardiseaserateandincludedthefollowinglevels:0level(0%),firstlevel(1%-33%),secondlevel(34%-66%),thirdlevel(67%-99%),andfourthlevel(100%).A

7、nddiseaseresistanceevaluationresultsintwoconsecutiveyearsshowedapplyingtherelativeareaunderthediseaseprogresscurveproduced100%consistentresultsbetweenyears.[Conclusion]Thepresentstudyprovidespotentiallyusefuldataforimprovingthetechniquesusedtoevaluatecottonvarietydiseaseresistance,and

8、mayst