h2s對(duì)兔離體心臟心率的影響

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1、H2S對(duì)兔離體心臟心率的影響作者：陳立鋒徐濤范凌趙蘭平陳少峰溫曉競(jìng)張曉云馬建偉尹萬斌【摘要】目的：探討硫化氫（H2S）對(duì)兔離體心臟心率的影響。方法：用標(biāo)準(zhǔn)微電極細(xì)胞內(nèi)記錄技術(shù)，觀測(cè)硫化氫對(duì)兔右心房心肌細(xì)胞動(dòng)作電位頻率的影響。觀測(cè)指標(biāo)：自發(fā)放電頻率(rateofpacemakerfiring，RPF)。結(jié)果：(1)用200μmol/LNaHS灌流時(shí)，和正常對(duì)照組相比，RPF顯著減?。≒<0.01）。(2)400μmol/LNaHS與對(duì)照組比較，RPF顯著減?。≒<0.01）。和200μmol/LN

2、aHS相比，RPF顯著減?。≒<0.01）。(3)以上使RPF減小的效應(yīng)在NaHS灌流3～5min出現(xiàn)，10min時(shí)已經(jīng)有顯著減小（P<0.01），25～30min達(dá)到高峰。結(jié)論：H2S可濃度依賴性的影響兔離體心臟的心率?！娟P(guān)鍵詞】心率；電生理；硫化氫；心臟；兔Hydrogensulfide(H2S)hasbeenprovedtobethethirdendogenoussignalinggasotransmitter,besidesnitricoxide(NO)andcarbonmonoxide

3、(CO).Itshowsimportantphysiologicalfunctions,involvinginthelongtermpotentiationinthehippocampusinthecentralnervoussystem,relaxinggastrointestinaltractsmoothmuscles[1,2].H2Shasbeenalso11foundtoplaymanyregulatingfunctionsincardiovascularsystem,andplayacertai

4、neffectinmanycardiovasculardiseases[3].Buttheeffectonheartratehasntbeendeeplyreseached.ByusingstandardintracellularmicroelectrodetechniquetorecordAps,hydrogensulfidewasusedtoinvestigatetherateofpacemakerfiringofleftatrium.Theaimofthestudywastoobservetheef

5、fectsofhydrogensulfideonheartrateofrabbitheartsinvitro,elucidatethemechanism(s)involved,provideelectrophysiologicproofforthenosogenesisofsomecardiovasculardiseasesandprovideresearchdirectionsforthedevelopmentofcardiovasculardrugs.1MATERIALSANDMETHODS1.1Ani

6、malandReagentsRabbitsofeithersex(2.2±0.2)kg,providedbyExperimentAnimalCenterofHeBeiNorthUniversity.NaHSwaspreparedwithHClandNa2Saftertheexperimentbegan.1.2SpecimenPreparation11Rabbitswerekilledwithasingleblowontheheadandtheheartswerequicklyremovedandplaced

7、inwarm(36.0±0.5)℃improvedLockesolution(NaCl0.157mol/L,KCl0.56×10-2mol/L,CaCl0.21×10-2mol/L,NaHCO30.18×10-2mol/Landglycose0.56×10-2mol/L).Itwasoxygenatedwith95%O2and5%CO2andmaintainedat（36.0±0.5）℃withpHof7.40±0.03.Therightatriummyocytes(includingsinuatrialn

8、ode)werecutfromtherightatriumandthenpinnedonathinsilicondiscatthebottomofaperfusionchamber.Thepreparationswereperfused(5mL/min)withwarm(36.0±0.5)℃improvedLockesolution.Itwasoxygenatedwith95%O2and5%CO2andmaint