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1、馬齒莧總黃酮抗小鼠缺氧作用及其機(jī)制研究論文董立巍，王萬(wàn)銀，岳義田，李敏【關(guān)鍵詞】,馬齒莧［摘要］目的：篩選馬齒莧抗缺氧作用有效成分，并對(duì)篩選出的有效成分進(jìn)行作用機(jī)制的研究。方法：馬齒莧抗缺氧作用有效成分篩選：將70只小鼠隨機(jī)分為總黃酮組、總生物堿組、多糖組、酸性成分組、堿性成分組、人參總皂苷組（陽(yáng)性對(duì)照）和生理鹽水組（陰性對(duì)照），每組10只。各組小鼠予以相應(yīng)藥物灌胃1周后，進(jìn)行常壓缺氧暴露生存時(shí)間實(shí)驗(yàn)，篩選出馬齒莧抗缺氧作用的有效成分。馬齒莧總黃酮抗缺氧機(jī)制研究：將90只小鼠隨機(jī)分為總黃酮組、人參總皂苷組和生理鹽水組.freelRNA的表達(dá)水平。結(jié)果：與生理鹽水組比較，馬齒莧總黃酮組小鼠缺
2、氧暴露生存時(shí)間明顯延長(zhǎng)；馬齒莧總黃酮組小鼠不同缺氧時(shí)段腎臟和大腦皮質(zhì)EPOmRNA的表達(dá)水平明顯增加，血漿EPO、紅細(xì)胞計(jì)數(shù)和血紅蛋白含量亦明顯升高。結(jié)論：總黃酮是馬齒莧抗缺氧作用的有效成分，其延長(zhǎng)小鼠缺氧生存時(shí)間的機(jī)制可能是促進(jìn)EPO的表達(dá)以及紅細(xì)胞和血紅蛋白的生成。［關(guān)鍵詞］馬齒莧;黃酮;缺氧;血紅蛋白類(lèi);紅細(xì)胞生成素EffectsofflavonesextractedfromPortulacaoleraceaonabilityofhypoxiatoleranceinmiceanditsmechanismABSTRACTObjective:Toidentifyantihypoxiain
3、gredientsextractedfromPortulacaoleraceaandtofindoutthepossiblemechanismofitsantihypoxiaactions.Methods:Seventymicelydividedintosevengroupsalsaline),ginsenosidestreated,polysaccharidetreated,acidicponentstreated,basicponentstreated,alkaloidstreatedandflavonestreatedgroups,andtheingredientsofpolysac
4、charide,acidicponents,basicponents,alkaloidsandflavonesPortulacaolerace.Themiceineachgroupeofmiceinhypoxicconditionsicealsaline),ginsenosidestreatedandflavonestreatedgroups.Themiceineachofthese3groupsoglobin(Hb)concentrationandhematocrit(HCT)inmiceined.Theplasmaerythropoietin(EPO)levelsofmiceelink
5、edimmunosorbentassay(ELISA)andtherelativevaluesofEPOmRNAinrenaltissueandpalliumofmiceinedbyreversetranscriptasepolymerasechainreaction(RTPCR).Results:ThesurvivaltimeofmiceinhypoxicconditionsinflavonestreatedgroupaEPOlevelandtherelativevaluesofEPOmRNAinrenaltissueandpalliumofmicePortulacaoleraceaar
6、eflavonesandtheantihypoxiaeffectsmaybeobtainedbyimprovingtheexpressionlevelofEPOandacceleratingthegenerationsoferythrocyteandHb.KEY］.第3版.北京:人民衛(wèi)生出版社,2002.15101512.8PriyadarshiA,PeriyasamyS,BurkeTJ,etal.Effectsofreductionofrenalmassonrenaloxygentensionanderythropoietinproductionintherat［J］.KidneyInt
7、,2002,61(2):542546.9BernaudinM,BellailA,MartiHH,etal.NeuronsandastrocytesexpressEPOmRNA:oxygensensingmechanismsthatinvolvetheredoxstateofthebrain［J］.Glia,2000,30(3):271278.10ChikumaM,MasudaS,KobayashiT,etal.Tissu
