資源描述：
《flavopiridol對eca9706細胞株放射增敏作用的體外研究》由會員上傳分享，免費在線閱讀，更多相關內(nèi)容在學術論文-天天文庫。
1、carcinomacelllines)[101，andwithstronginhibitoryeffectonthegrowthofhumanJuvesarcomacellline[¨1．While，themostcommonesophagealcancerinChinaissquamouscellcarcinoma，itisnotyetreportedthatwhetherFlavopiridolhaveradiosensitivity—enchancingeffectonEca9706cellline．Therefore，this
2、experimentculturedEta9706cellsinvitro，treatedthecellswithFlavopiridolor／andradiotherapy，testedthecellsurvivalrate，cellcycleandapoptosisrelatedsignalpathways，toinvestigatetheradiosensitivity—enchancingeffectofFlavopiridolonEca9706celllineanditsrelatedmechanism，providenew
3、ideasandmethodsforclinicaltreatmentofesophagealcancers．Purpose：Toinvestigatetheradiosensitivity-enchancingeffectofFlavopiridol(inhibitorsofthecellcycleproteinkinase—CDK)onEca9706cellline(humanesophagussquamouscellline)anditsrelatedmechanism·Methods：Assayedtheinhibitoryr
4、ateofgrowthandradiosensitivity‘enchancingrateofthecelllineunderFlavopiridoleffectbyMTTandcloningexperiment．Usingimmunocyto-chemistrytomeasuretheexpressionofsurvivin，caspase一3，P53．TestedtheapoptosisoftheEca9706celllineindifferentgroupsby玎邛范L．Results：1．ResultsofMTTandclon
5、ingexperimentshowedthatFlavopiridolcouldinhabitthesurvivalrateofEca9706cellssignificantly，relatedwithconcentrationandtime，theIC50was2．561xmol／L．2．ThedifferencesbetweenthesurvivalfractionsofcellstreatedwithFlavopiridolcombinedwithradiotherapyandsingleradiotherapyhadstati
6、sticalsignificance(P<0．05)．Manytargetclickmodel，thesurvivalcurvesfitting：thesensitizationenhancementratioofEca9706celllineR+O。1xlC50，R+0．2xlC50were1．2375and1．3456．3．Resultsofimmunocytochemistryshowedthatthepositiverateofcaspase一3inthetestgroupwas67％，haddifferenceswithth
7、eothergroups(P<0．05)；thepositiverateofsurvivininthetestgroupwas24％，haddifferenceswiththeothergroups(P<0．05)；thepositiverateofP53inthetestgroupwas31％，haddifferenceswiththeothergroups(P<0．05)．4．ResultsofTUNELshowedthatapoptosisindex(AI)ofdruggroupwas(35．8士1．94)％，AIofradia
8、tiongroupwas(47．1+1．71)％，AIofthetestgroupwas(68．3士2．24)％，AIofthecontrolgroupwas(5．14-0．71)％．4Conclusion：1．Flav
