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《2.2.27. Thin-layer chromatography(中英)》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在教育資源-天天文庫。
1、2.2.27.Thin-layerchromatography薄層色譜法Thin-layerchromatographyisaseparationtechniqueinwhichastationaryphaseconsistingofanappropriatematerialisspreadinauniformthinlayeronasupport(plate)ofglass,metalorplastic.Solutionsofanalytesaredepositedontheplatepriortodevelopme
2、nt.Theseparationisbasedonadsorption,partition,ion-exchangeoroncombinationsofthesemechanismsandiscarriedoutbymigration(development)ofsolutes(solutionsofanalytes)inasolventorasuitablemixtureofsolvents(mobilephase)throughthethin-layer(stationaryphase).薄層色譜法是一種分離技術(shù)，
3、這種技術(shù)采用由適當物質(zhì)作為固定相均勻涂布在玻璃、金屬或塑料的支持物上。在展開前，供試品液點樣在薄層板上。分離原理是供試品溶解在溶劑中或合適的混合溶劑中（流動相）在薄層上（固定相）通過吸附、分配、離子交換或以上機制共同作用移動（展開）來實現(xiàn)的。APPARATUS儀器Plates.Thechromatographyiscarriedoutusingpre-coatedplatesasdescribedunderReagents(Error!Nobookmarknamegiven.).薄層板色譜法是按照4.1.1試劑項下描述
4、的涂層板來進行的。Pre-treatmentoftheplates.Itmaybenecessarytowashtheplatespriortoseparation.Thiscanbedonebymigrationofanappropriatesolvent.Theplatesmayalsobeimpregnatedbyproceduressuchasdevelopment,immersionorspraying.Atthetimeofuse,theplatesmaybeactivated,ifnecessary,by
5、heatinginanovenat120°Cfor20min.薄板預處理：在分離前用適當?shù)娜軇﹣砬逑幢“濉？捎眠m當?shù)娜軇┫疵?。在進行展開、浸漬或噴霧顯色過程中要將薄板浸透。使用時，如果需要，要在120℃干燥箱中加熱20min活化薄板。Chromatographictankwithaflatbottomortwintrough,ofinert,transparentmaterial,ofasizesuitablefortheplatesusedandprovidedwithatightlyfittinglid.Forho
6、rizontaldevelopmentthetankisprovidedwithatroughforthemobilephaseanditadditionallycontainsadevicefordirectingthemobilephasetothestationaryphase.層析缸：使用適合薄層板大小的無活性、透明物質(zhì)制成的層析缸，并有嚴密的蓋子，底部平整，或有雙槽。水平展開的層析缸有一個裝展開劑的槽，并且額外有一個裝置用來引導展開劑到固定相。Micropipettes,microsyringes,calib
7、rateddisposablecapillariesorotherapplicationdevicessuitablefortheproperapplicationofthesolutions.微量加液器、微量注射器、有刻度的一次性毛細管：或其它適合于應用溶液的裝置。Fluorescencedetectiondevicetomeasuredirectfluorescenceortheinhibitionoffluorescence.熒光檢測裝置：用來測量直接熒光或熒光抑制Visualisationdevicesandr
8、eagents.Suitabledevicesareusedforderivatisationtotransfertotheplatereagentsbyspraying,immersionorexposuretovapourand,whereapplicable,tofacilitateheatingforvisualisati
