2.2.27. Thin-layer chromatography(中英)

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1、2.2.27.Thin-layerchromatography薄層色譜法Thin-layerchromatographyisaseparationtechniqueinwhichastationaryphaseconsistingofanappropriatematerialisspreadinauniformthinlayeronasupport(plate)ofglass,metalorplastic.Solutionsofanalytesaredepositedontheplatepriortodevelopme

2、nt.Theseparationisbasedonadsorption,partition,ion-exchangeoroncombinationsofthesemechanismsandiscarriedoutbymigration(development)ofsolutes(solutionsofanalytes)inasolventorasuitablemixtureofsolvents(mobilephase)throughthethin-layer(stationaryphase).薄層色譜法是一種分離技術(shù)，

3、這種技術(shù)采用由適當(dāng)物質(zhì)作為固定相均勻涂布在玻璃、金屬或塑料的支持物上。在展開前，供試品液點樣在薄層板上。分離原理是供試品溶解在溶劑中或合適的混合溶劑中（流動相）在薄層上（固定相）通過吸附、分配、離子交換或以上機制共同作用移動（展開）來實現(xiàn)的。APPARATUS儀器Plates.Thechromatographyiscarriedoutusingpre-coatedplatesasdescribedunderReagents(Error!Nobookmarknamegiven.).薄層板色譜法是按照4.1.1試劑項下描述

4、的涂層板來進行的。Pre-treatmentoftheplates.Itmaybenecessarytowashtheplatespriortoseparation.Thiscanbedonebymigrationofanappropriatesolvent.Theplatesmayalsobeimpregnatedbyproceduressuchasdevelopment,immersionorspraying.Atthetimeofuse,theplatesmaybeactivated,ifnecessary,by

5、heatinginanovenat120°Cfor20min.薄板預(yù)處理：在分離前用適當(dāng)?shù)娜軇﹣砬逑幢“??？捎眠m當(dāng)?shù)娜軇┫疵摗Ｔ谶M行展開、浸漬或噴霧顯色過程中要將薄板浸透。使用時，如果需要，要在120℃干燥箱中加熱20min活化薄板。Chromatographictankwithaflatbottomortwintrough,ofinert,transparentmaterial,ofasizesuitablefortheplatesusedandprovidedwithatightlyfittinglid.Forho

6、rizontaldevelopmentthetankisprovidedwithatroughforthemobilephaseanditadditionallycontainsadevicefordirectingthemobilephasetothestationaryphase.層析缸：使用適合薄層板大小的無活性、透明物質(zhì)制成的層析缸，并有嚴(yán)密的蓋子，底部平整，或有雙槽。水平展開的層析缸有一個裝展開劑的槽，并且額外有一個裝置用來引導(dǎo)展開劑到固定相。Micropipettes,microsyringes,calib

7、rateddisposablecapillariesorotherapplicationdevicessuitablefortheproperapplicationofthesolutions.微量加液器、微量注射器、有刻度的一次性毛細管：或其它適合于應(yīng)用溶液的裝置。Fluorescencedetectiondevicetomeasuredirectfluorescenceortheinhibitionoffluorescence.熒光檢測裝置：用來測量直接熒光或熒光抑制Visualisationdevicesandr

8、eagents.Suitabledevicesareusedforderivatisationtotransfertotheplatereagentsbyspraying,immersionorexposuretovapourand,whereapplicable,tofacilitateheatingforvisualisati