二甲雙胍對(duì)人肝癌細(xì)胞Bel-7402增殖與凋亡的影響-論文.pdf

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1、14四川生理科學(xué)雜志2014；36(1)Anti—aginghealthcareeffectofPu-erteaOild-galactose-inducedsenescentmiceiZhuangQiu—rong，YuHai—jiang，ZhengYou，LiJin-fang，SuJuan，QinYan△(1．Class2Batch2008ofMedicine，YunnanDaIi671000；2．DepartmentofPyhsiologyandPathophsiology，SchoolofBasicMedicine，DaLiUniversity

2、，YunnanDaLi671000)Al~tractObjeefi~：Tostudytheanti-agingactivitypu-erteainagedmiceinducedbyD-galactose_Methods：Thirtymiceweredivid—edinto3groups：thecontrolgroup，theagingmodelgroupandthepu-erteagroup(60mg·rnl一)．ThemicewereadministratedD-g~amtose(0．25ml·10g一)byhypodermicinjection

3、everyday{or6weeks．Fromsecondweek，thenficeinpwerteagroupwereadrni~stmtedpuertea(60rng·ml)intragastricallyatthesainetime{or4weks．Thelevelofsupero菇de~smumse(S0D)，andmalonaldehyde(ⅣⅡ)A)inserunlandlivertissuesweredetermined；theorganm~cesofthekidney，splenand1iverofmicewereobserved．R

4、esults：Comparedwiththecontrolgroup，theserulTlS(1)Dlevelsignificantlydecreasedintheagingmodelgroup(P％0．05)，theliverMI)Alevelsignificantlyin—creasedintheagingmodelgroup(P

5、velssignificantlyincreasedinthepu-erteagroup(p<0．05)，theMDAlevelsignificantlydmreasedinthepuerteagroup(P

6、；Anti-aging；Visceralindex二甲雙胍對(duì)人肝癌細(xì)胞Bel一7402增殖與凋亡的影響郭艷紅黃文君田晶辛敏△(桂林醫(yī)學(xué)院生理教研室，廣西桂林541004)摘要目的：研究二甲雙胍抑制人肝癌細(xì)胞Bel一7402增殖及調(diào)亡的影響，并初步探討其機(jī)制。方法：體外培養(yǎng)人肝癌細(xì)胞株Bel一74O2，以不同濃度的二甲雙胍(1OmM、20mM、40mM)干預(yù)細(xì)胞48h。采用MTT法檢測(cè)藥物對(duì)細(xì)胞增殖的影響；采用H0一eehst33258熒光染色法和流式細(xì)胞術(shù)檢測(cè)細(xì)胞凋亡；Real-timePCR檢測(cè)oaspase-3、bax和6c￡一2的mRNA水平

7、表達(dá)。結(jié)果：與對(duì)照組相比，不同濃度的二甲雙胍作用于Bel一7402細(xì)胞48h后，MTT結(jié)果顯示對(duì)細(xì)胞增殖有明顯抑制作用(P<0．05)；Hoechst33258熒光染色法顯示凋亡細(xì)胞明顯增多；流式細(xì)胞術(shù)分析顯示二甲雙胍可明顯誘導(dǎo)Be1-7402細(xì)胞凋亡，晚期凋亡率分別為3．76，8．96和18．67；caspase-3、bax和bcl一2的mRNA表達(dá)水平均上調(diào)(P

8、甲雙胍；肝細(xì)胞癌；凋亡二甲雙胍屬于雙胍類口服降糖藥，也是臨床治療2型糖尿用RPMI1640培養(yǎng)基、雙抗及胎牛血清均購(gòu)自美國(guó)